自身免疫性肝病患者外周血 Th17与 Treg 细胞及其相关因子表达的研究

目的 研究自身免疫性肝病（AILD）患者外周血中辅助性 T 细胞17（Th17）、调节性 T 细胞（Treg 细胞）及其细胞因子白细胞介素-17（IL-17）、转化生长因子-β（TGF-β）的表达情况。方法 选择46例 AILD 患者，分为自身免疫性肝炎患者（AIH 组）和原发性胆汁性肝硬化患者（PBC 组），采用流式细胞术检测 AIH 组、PBC 组和对照组外周血单个核细胞中 Th17和 Treg 细胞的比例；应用酶联免疫吸附法（ELISA）检测血清中 Th17的相关因子 IL-17和 Treg的相关因子 TGF-β含量。结果 AIH 组和 PBC 组患者 Th17细胞比例高于对照组（P ＜0．01），Treg 细胞显著低于对照组（P ＜0．05）；AIH 组和 PBC 组患者 IL-17的含量较对照组升高（P ＜0．05），血清 TGF-β的含量较对照组降低（P＜0．01）。结论 在 AILD 患者中，Th17、Treg 细胞的变化可能在 AILD 发病机制中发挥重要作用。

Studies on the population of Th17 and Treg cells and the expression of the correlated cytokines in autoimmune liver disease patients

Objective To examine the serum levels of interleukin（IL）-17,Transforming growth factor （TGF-β）and the proportion of peripheral of Thl7 cells and Treg cells in patients with Autoimmune Liver Disease （AILD）.Methods Flow cytometric assay were used to analyse the percentages of Th17 and Treg cells in autoimmune hepatitis （AIH） and primary biliary cirrhosis （PBC）patients.The levels of IL-17 and TGF-βin plasma were measured by enzyme linked immunosorbent assay（ELISA）.Results The percentages of Th17 cells in patients with AIH PBC was significantly higher than that of normal controls（P 〈0.01）and the Treg percentages decreased obviously（P 〈0.05）.The serum levels of IL-17 were higher than controls（P 〈0.05）,serum concentrations of TGF-βwere lower than controls （P 〈0.01）. Conclusion Th17 and Treg cells may play an important role in the pathogenesis of AILD,which is one of probably caused by the body＇s immune system to balance the tendency of proinflammatory direction.