流式细胞技术检测血小板相关抗体在ITP诊断中的应用研究

目的探讨流式细胞技术检测血小板相关抗体在ITP诊断中的意义。方法选择2012年5月至2013年6月在我院血液科住院的30例ITP患者为观察组,同期本院健康体检正常者30例为对照组,采用流式细胞技术检测两组对象的外周血血小板相关抗体PAIg（PAIgG、PAIgM、PAIgA）水平。结果 FCM检测观察组患者血小板抗体,PAIgG阳性率为70%,平均荧光强度为（25.15±2.61）,对照组平均荧光强度为（5.09±0.54）,两组比较有显著性差异（P〈0.05）;观察组PAIgM阳性率为80%,平均荧光强度（27.28±2.25）,对照组平均荧光强度为（5.18±0.58）,两组比较有显著性差异（P〈0.05）;观察组PAIgA阳性率为30%,平均荧光强度为（6.28±0.65）,对照组平均荧光强度为（5.98±0.68）,两组比较差异无统计学意义（P〉0.05）。结论流式细胞仪检测血小板相关抗体在ITP诊断中具有重要的临床意义。

Clinical Value of Platelet-Associated Ig Examination for ITP Diagnosis by FCM

Objective to investigate the significance of platelet-assosiated Ig by flow cytometry （FCM）in the diag-nosis of idiopathic thrombocytopenic pupura.Methods Took the Control study,and selected the types of non- ITP thrombocytopenic patients with various indicators as a reference object FCM was used for detection of platelet associat-ed antibody in 30 patients with ITP and 30 healthy control.Results Using the FCM detection of platelet antibodies in patients with ITP,Positive rate of PAIgG was 70%,the average of fluorescence intensity was（25.15 ± 2.61）,and the average of fluorescence intensity in normal control group was （5.09 ± 0.54）,there was a significant difference between the two （P 〈0.05）;Positive rate of PAIgA was 80%,the average of fluorescence intensity was（27.28±2.25）,and the average of fluorescence intensity in normal control group was（5.18±0.58）,there was a significant difference between the two （P 〈0.05）;Positive rate of PAIgG was 30%,the average of fluorescence intensity was 6.28±0.65,and the av-erage of fluorescence intensity in normal control group was 5.98±0.68,there wasn’t a significant difference between the two （P 〈0.05）;Conclusion Flow cytometry detection of platelet-associated antibodies have important significance for the diagnosis of ITP.