7种抗蠕虫药物的体外抗华支睾吸虫作用

目的观察吡喹酮、三苯双脒、左旋咪唑、蒿甲醚、青蒿琥酯、阿苯达唑和甲苯达唑体外对华支睾吸虫成虫的作用。方法 70只大鼠于感染华支睾吸虫囊蚴(50～100个/只)5～7周后解剖,从胆总管内采集华支睾吸虫成虫,亨氏盐平衡溶液培养。取24孔培养板,每孔放置华支睾吸虫3～4条,加入不同浓度的上述药物,于药物处理后1、4、24、48和72 h,在倒置显微镜下观察成虫的活动和形态变化。结果吡喹酮可迅速减弱华支睾吸虫的活动,使口吸盘丧失吸附皿壁能力,虫体蜷缩、皮层出现空泡。吡喹酮对华支睾吸虫的最低致死浓度为0.1μg/ml。三苯双脒0.5、1和10μg/ml组虫体接触药物后迅速麻痹伸直呈松弛状。三苯双脒对华支睾吸虫的最低致死浓度为0.05μg/ml。左旋咪唑10和20μg/ml组华支睾吸虫的活动经药物作用后逐渐减弱,虫体松弛,但48h后,大部分虫体和口吸盘明显恢复活动。左旋咪唑的浓度高达50μg/ml时,虫体立即伸直麻痹,其表现与三苯双脒组相仿。蒿甲醚和青蒿琥酯10μg/ml和50μg/ml组虫体和口吸盘的活动减弱,虫体收缩,继则松弛,体表出现空泡,培养72 h后两组均有半数以上虫体死亡。阿苯达唑和甲苯达唑10μg/ml和5...

In vitro effect of seven anthelmintic agents against adult Clonorchis sinensis

Objective To observe the in vitro effect of praziquantel,tribendimidine,levamisole,artemether,arte-sunate,albendazole and mebendazole against adult Clonorchis sinensis.Methods Seventy rats infected with 50-100 C.sinensis metacercariae for 5-7 weeks were euthanized,and adult C.sinensis were collected from the common bile duct.Three to four worms were placed in each well of a 24-well falcon plate,and treated by Hanks' balanced salt solution-20% calf serum containing aforementioned drugs at various concentrati...