| 棘阿米巴土壤分离株CB/S1内共生细菌的16S rDNA序列分析 |
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| 作者姓名： | Xuan YH Cui CQ Zheng SZ |
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| 作者单位： | 延边大学基础医学院免疫学与病原生物学教研部; |
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| 基金项目： | 吉林省教育厅科技项目(No.2006-6)~~ |
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| 摘 要： | 用地衣红-卡红染色进行共生菌的形态观察,鉴定棘阿米巴CB/S1内共生细菌。克隆内共生细菌的16S rDNA基因,进行基因序列分析。结果表明,经地衣红-卡红染色棘阿米巴CB/S1内共生细菌呈黑色和棒状,在胞质内不规则分布。棘阿米巴CB/S1内共生细菌的16S rDNA基因长1534bp,与类亚洲嗜阿米巴杆菌(Candidatus Amoebophilus asiaticus 5a2)和韩国棘阿米巴分离株KA/E21内共生细菌的16S rDNA基因的同源性均为98%。进化树分析表明,棘阿米巴CB/S1内共生细菌与韩国棘阿米巴KA/E21内共生细菌、类亚洲嗜阿米巴杆菌、黑脚硬蜱内共生细菌和伯恩蚜小蜂内共生细菌等细菌构成单系。
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| 关 键 词： | 棘阿米巴 16SrDNA 内共生细菌 类亚洲嗜阿米巴杆菌 |
Sequence analysis of 16S rDNA gene of endosymbiont of Acanthamoeba sp. CB/S1 isolated from soil |
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| Xuan YH,Cui CQ,Zheng SZ.Sequence analysis of 16S rDNA gene of endosymbiont of Acanthamoeba sp. CB/S1 isolated from soil[J].Chinese Journal of Parasitology and Parasitic Diseases,2011,29(2):98-100. |
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| Authors: | Xuan Ying-hua Cui Chun-quan Zheng Shan-zi |
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| Institution: | XUAN Ying-hua,CUI Chun-quan,ZHENG Shan-zi(Department of Pathogenic Biology,College of Basic Medicine,Yanbian University,Yanji 133000,China) |
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| Abstract: | The endosymbiont of Acanthamoeba sp.CB/S1 was identified by orcein-carmine staining and 16S rDNA sequence analysis.The endosymbiont bacteria were rod-shaped and darkly stained,and irregularly localized within the cy-toplasm.The length of the 16S rDNA was 1 534 bp and its DNA sequence was closely related to those of Candidatus Amoebophilus asiaticus and Acanthamoeba sp.KA/E21 with 98% homology.Phylogenetic analysis showed that the end-osymbiont of CB/S1,the endosymbiont of KA/E21,Candidatus Amoebophilus asia... |
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| Keywords: | Acanthamoeba 16S rDNA Endosymbiont Candidatus Amoebophilus asiaticus |
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