| 湖北钉螺凝集素的提取及其活性研究 |
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| 引用本文： | Peng HM,Li CP,Liu H,Zhao JS,Zhou SL. 湖北钉螺凝集素的提取及其活性研究[J]. 中国寄生虫学与寄生虫病杂志, 2011, 29(2): 130-133 |
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| 作者姓名： | Peng HM Li CP Liu H Zhao JS Zhou SL |
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| 作者单位： | 皖南医学院;江苏省建湖县中医院; |
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| 基金项目： | 安徽省自然科学基金(No.11040606M214); 安徽省教育厅自然科学基金(No.kj2009B152)~~ |
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| 摘 要： | 目的探讨湖北钉螺(Oncomelania hupensis)体内凝集素的提取方法及其凝集活性。方法用20%～100%硫酸铵对钉螺蛋白进行梯度分级沉淀,检测不同沉淀对兔红细胞的凝集活性。分别用2.5 mg/ml D-半乳糖、D-果糖、D-葡萄糖、蔗糖、麦芽糖和乳糖等6种糖溶液对有凝集活性的沉淀进行倍比稀释,测定其红细胞凝集活性的变化。并测定不同温度(25～90℃)和pH值(3.0～10.0)对钉螺凝集素凝集活性的影响。结果硫酸铵分级沉淀法提取钉螺凝集素时,20%～40%饱和梯度所得的沉淀具有较高的凝集活性。其凝集活性能被半乳糖和乳糖抑制。当pH值为7.0时钉螺凝集素活性最高。30～70℃水浴时,随温度的增加其凝集活性降低,高于80℃时无凝集活性。结论钉螺体内存在半乳糖和乳糖凝集素,其凝集活性受温度和酸碱度的影响。
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| 关 键 词： | 湖北钉螺 凝集素 提取 凝集活性 |
Extraction of agglutinin from Oncomelania hupensis and its haemagglutination activity |
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| Peng Huai-ming,Li Chao-pin,Liu Hui,Zhao Jin-song,Zhou Shu-lin. Extraction of agglutinin from Oncomelania hupensis and its haemagglutination activity[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2011, 29(2): 130-133 |
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| Authors: | Peng Huai-ming Li Chao-pin Liu Hui Zhao Jin-song Zhou Shu-lin |
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| Affiliation: | PENG Huai-ming1,2,LI Chao-pin1,LIU Hui1,ZHAO Jin-song1,ZHOU Shu-lin1 (1 Wannan Medical College,Wuhu 241000,China,2 Jianhu Hospital of Traditional Chinese Medicine,Jianhu 224700,China) |
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| Abstract: | Objective To explore the extraction methods of agglutinin from Oncomelania hupensis snail and study its haemagglutination activity.Methods Protein obtained by ammonium sulfate fractionation precipitation with 20%-100% saturation of ammonium sulfate.Its haemagglutination activity was determined by rabbit erythrocytes.The precipit-ation which could agglutinate rabbit erythrocytes was diluted with 2.5 mg/ml D-galactose,D-fructose,D-glucose,saccharose,maltose and lactose,respectively,and then their haemagglutin... |
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| Keywords: | Oncomelania hupensis Agglutinin Extraction Haemagglutination activity |
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