结核分枝杆菌多重感染的初步研究

目的 鉴定结核分枝杆菌多重感染的存在.方法 从上海市疾病预防控制中心结核病参比实验室保存的菌株中,选择1999年至2004年在同一抗结核疗程中前后2次分离菌株的耐药表型改变的患者为研究对象.挑选并活化入选病例的首次分离株,分离培养单菌落.利用可变数目串联重复序列多态性分型(VNTR)方法,对每个分离株的30个单菌落进行基因型分析.结果 在22例入选病例中,2例患者的首次分离株中存在2种不同基因型的菌株.2种不同基因型菌株在单菌落中占的比例分别是24:6和29:1.其余20例患者分离株各自的30个单菌落的基因型相同.结论 上海地区结核病患者中存在结核分枝杆菌的多重感染.

Multiple infections of Mycobacterium tuberculosis in treated patients

OBJECTIVE: To identify multiple infections of Mycobacterium tuberculosis in tuberculosis patients. METHODS: From the clinical isolates of M. tuberculosis during 1999 to 2004 stored at Shanghai Municipal Center for Disease Control and Prevention, cases who had two isolates with different drug resistance patterns in the same treatment episode were selected. The first isolate from the selected patients was inoculated onto Middlebrook 7H11 agar plates, and 30 single colonies from each isolate were picked and genotyped by the 7 loci Variable Number Tandem Repeat (VNTR) method. RESULTS: Two out of 22 isolates had two different genotypes among their 30 single colonies, and the proportions of the two genotype colonies were 24:6 and 29:1. The other 20 isolates showed identical genotype pattern among their 30 single colonies. CONCLUSION: Multiple infections of M. tuberculosis occurred in tuberculosis patients in Shanghai.