Higher-order organization and compartmentalization of satellite DNA PIM357 in species of the coleopteran genus Pimelia |
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Authors: | Joan Pons Carlos Juan Eduard Petitpierre |
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Institution: | 1. Laboratori de Genètica, Departament de Biologia, Universitat de les Illes Balears, 07071, Palma de Mallorca, Spain 2. Division of Insect Biology, ESPM, University of California, Wellman Hall 201, Berkeley, CA, 94720-3112, USA 3. Departament de Recursos Naturals, Institut Mediterrani d'Estudis Avan?ats (CSIC-UIB), C/.Miquel Marqués, 21, 07190, Esporles, Balearic Islands, Spain
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Abstract: | The PIM357 satellite DNA family is present in 26 Pimelia taxa (Tenebrionidae, Coleoptera) with endemic congeneric species from the Canary Islands showing higher interrepeat variability
than continental ones. In this paper, we compare the repetitive DNA sequences of a Canarian species that has distinct subfamilies
of repeat units, P. radula ascendens, with another without such subfamilies, P. sparsa sparsa. The chromosomal localization of the repeat units and the comparison of the variability of randomly cloned monomers to the
one estimated by comparing repeat units from dimers and trimers suggest the absence of satellite subfamilies in P. sparsa sparsa. Hence, the repeat units of this species seem to be uniformly and randomly distributed throughout all chromosomes out of
one chromosomal pair. On the contrary, P. radula ascendens shows four divergent subfamilies of repeat units supported by several diagnostic nucleotide substitutions. These subfamilies
seem to form four distinct repeat units: monomer subfamily 1, monomer subfamily 4 and two higher-order units (dimer linking
subfamily 1 and 4, and dimer linking subfamily 2 and 3). Moreover, monomers of subfamily 1 are present in three chromosomal
pairs only. We discuss the effect of different potential factors acting in the concerted evolution and the genomic organization
of stDNA sequences in these taxa.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | chromosome Coleoptera satellite DNA |
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