胶原结合分析试验在血管性血友病检测中的应用

目的探讨胶原结合分析试验在血管性血友病(vWD)检测中的应用前景.方法应用ELISA 法作vWF胶原结合分析(vWFCBA),对该方法的敏感性、重复性作检测,并同时使用四种检测vWD的方法检测正常献血员、vWD患者、及其它出血性疾病患者的血浆vWF功能. 结果 vWD患者血浆vWFCBA水平明显低于正常人及其它出血性疾病患者(P<0.001);四种检测血管性血友病的试验其结果在vWD患者组与正常献血员组和其它出血性疾病组比较均有显著差异(P<0.01);正常献血员组与其它出血性疾病组比较无统计学差异(P>0.05).四种检测方法与各型vWD患者诊断总的符合率分别为vWFAg 85.7%,vWFRcof 76.2%,RIPA 80.9%,vWFCBA 95.2%. 结论 ELISA法检测vWFCBA操作简便,方法特异性、敏感性和重复性均较好,应用该方法可为vWD的诊断和分型能提供依据,在常规vWD的诊断分型中可替代vWFRcof和RIPA,值得临床推广应用.

Application of Collagen-binding Assay for von Willebrand Disease

Objective To explore the application of collagen-binding assay for von Willebrand disease (vWD). Methods We investigated the sensitivity and reproducibility of collagen-binding assays (CBA) of vWF using the enzyme-linked immunosorbent assay (ELISA) technique and compared CBA with 3 other assays for their ability to detect vWD. Results It was found that the vWF:CBA level of plasma from patients with different types of vWD is lower than that from healthy donors, hemophilia and other bleeding disorders (P<0.001). In general, the four assays have the capability to identify vWD from normal and other bleeding disorders, and can be used for diagnosing vWD. The concordance rates of the four assays on vWD are vWF:Ag 85.7%, vWF:Rcof 76.2%, RIPA 80.9%, vWF:CBA 95.2% respectively. Conculusion vWF:CBA measured by ELISA is highly sensitive, specific and reproducible, and easy to operate. The measurement of the functional activity of vWF by vWF:Rcof or RIPA can be replaced by the more reliable vWF:CBA.