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| 一株产类胡萝卜素海洋细菌的分离及系统分类学研究 | |
| 引用本文： | 张文俊,田宏,杨桥,焦炳华,吴文惠,蒋平,缪辉南,包斌. 一株产类胡萝卜素海洋细菌的分离及系统分类学研究[J]. 中华航海医学与高气压医学杂志, 2009, 16(5). DOI: 10.3760/cma.j.issn.1009-6906.2009.05.005 |
| 作者姓名： | 张文俊 田宏 杨桥 焦炳华 吴文惠 蒋平 缪辉南 包斌 |
| 作者单位： | 1. 上海海洋大学食品学院,上海,201306 2. 赤峰制药股份有限公司 3. 第二军医大学生物化学与分子生物学教研室 |
| 基金项目： | 国家留学同国人员基金，上海浦江人才基金，中国博士后科学基金 |
| 摘 要： | 目的 对一株分离自我国东海海域160 m深处产类胡萝卜素的海洋细菌进行形态学、生理生化特性及分子水平的系统分类学研究.方法 通过细菌形态学特征观察、生长条件、生理生化特征测定、全细胞脂肪酸成分分析、16S rRNA序列扩增、测序、比对及构建系统发育进化树,研究了该菌株的系统分类学特性;并通过高效液相色谱对该菌株的类胡萝卜素产出特性进行了分析.结果 该菌株与考克氏菌属(Kocuria sp.)相似度最高,顺-7-棕榈油酸/顺-6-棕榈油酸为该菌株的主要脂肪酸组分.结论 该菌株属于考克氏菌属,暂定名为考克氏菌CAR-red(Kocuria sp.CAR-red)株. |
| 关 键 词： | 类胡萝卜素 考克氏菌 海洋细菌 系统分类学 |
Isolation and taxonomic study on a carotenoid -producing marine bacterial strain |
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| ZHANG Wen-jun,TIAN Hong,YANG Qiao,JIAO Bing-hua,WU Wen-hui,JIANG Ping,MIAO Hui-nan,BAO Bin. Isolation and taxonomic study on a carotenoid -producing marine bacterial strain[J]. Chinese Journal of Nautical Medicine and Hyperbaric Medicine, 2009, 16(5). DOI: 10.3760/cma.j.issn.1009-6906.2009.05.005 | |
| Authors: | ZHANG Wen-jun TIAN Hong YANG Qiao JIAO Bing-hua WU Wen-hui JIANG Ping MIAO Hui-nan BAO Bin |
| Abstract: | Objective To perform taxonomic studies on a carotenoid -producing marine bacterium isolated from the mud sample at a depth of 160 m in East China Sea. Methods Taxonomic studies were performed with phenotypic and BIOLOG analysis, 16 S rRNA gene sequencing and blast analysis, and fatty acid component analysis. Carotenoids produced by this bacterial strain were extracted and analyzed with high-performance liquid chromatography (HPLC). Results CAR -red strain showed high similarity with the genus of kocuria sp. The predominant fatty acid component was 16:1 w7c/16:1 w6c based on GC analysis. Conclusions A CAR-red strain belonged to the genus of kocuria sp. , and kocuria CAR-red was proposed as the name for this strain on the basis of the taxonomic data. |
| Keywords: | Carotenoids Kocuria sp Marine bacteria Taxonomic study |
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