同型半胱氨酸诱导人脐静脉内皮细胞MCPIP表达 |
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引用本文: | 吴柳松,冯永怀,钱民章. 同型半胱氨酸诱导人脐静脉内皮细胞MCPIP表达[J]. 遵义医学院学报, 2014, 0(2): 183-187 |
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作者姓名: | 吴柳松 冯永怀 钱民章 |
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作者单位: | [1]遵义医学院生物化学教研室,贵州遵义563099; [2]遵义医学院附属医院血液内科,贵州遵义563099 |
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基金项目: | 遵义医学院招标项目(NO:F-613);遵义医学院研究生创新计划项目(NO:CX201308). |
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摘 要: | 目的 探讨同型半胱氨酸是否可以诱导人脐静脉内皮细胞中MCPIP的表达.方法 体外培养并鉴定人脐静脉内皮细胞;分别用10、50、100、200、500 μmol/L Hcy与HUVECs孵育24 h;用100 μmol/L Hcy与HUVECs分别孵育1、2、4、8、12、24 h;Real-time PCR检测MCPIP mRNA的表达,Western blot检测MCPIP蛋白质的表达.结果 Real-time PCR和Western blot分析结果表明,在所设置浓度下,Hcy均可诱导HUVECs中MCPIP的表达(P<0.05),其中以100 μmol/L Hcy作用24 h时MCPIP表达量最多;100 μmol/l Hcy与细胞作用2h时后MCPIP表达与对照组相比即有明显增高(P<0.05),并存在时间依赖效应.结论 Hcy可诱导HUVECs中MCPIP的表达,这可能与它致As的机理有关.
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关 键 词: | 同型半胱氨酸 人脐静脉内皮细胞 |
Homocysteine induces monocyte chemoattractant protein-1 induced protein expression in human umbilical vein endothelial cells |
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Affiliation: | Wu Liusong , Feng Yonghuai, Qian Minzhang (1. Department of Biochemistry, Zunyi Medical University, Zunyi Guizhou 563099, China;2. Department of He- matology,The Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099, China) |
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Abstract: | Objective To investigate whether homoeysteine (Hcy) induced Monoeyte chemoattractant protein - 1 induced protein (MCPIP) expression in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured and identified in vitro. 2. HUVECs were treated with different concentrations of Hey (10,50,100, 200 and 500 μmoL/L) for 24 h and were stimulated with Hey ( 100 μmol/L) at differenttimes ( 1,2,4,8,12,24 h). The MCPIP mRNA and protein expressions were detected by real - time fluorescence quantitative PCR ( Real -time PCR) and western blotting assay, respectively. Results Different concentrations of Hey induced MCPIP expression in HUVECs with the significantly highest MCPIP production 24 h after Hey (100 μmol/L) treatment. Hey ( 100 μmol/L) time - dependently induced HUVECs MCPIP expression from Hey treatment for 2 h (P 〈 0. 05 ). Conclusion Hey induces MCPIP expression in HUVECs, which might be related to the pathogenesis of ath- erosclerosis. |
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Keywords: | homocysteine human umbilical vein endothelial cells monocyte chemoattractant protein - 1 in-duced protein |
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