同型半胱氨酸诱导人脐静脉内皮细胞MCPIP表达

目的 探讨同型半胱氨酸是否可以诱导人脐静脉内皮细胞中MCPIP的表达.方法 体外培养并鉴定人脐静脉内皮细胞;分别用10、50、100、200、500 μmol/L Hcy与HUVECs孵育24 h;用100 μmol/L Hcy与HUVECs分别孵育1、2、4、8、12、24 h;Real-time PCR检测MCPIP mRNA的表达,Western blot检测MCPIP蛋白质的表达.结果 Real-time PCR和Western blot分析结果表明,在所设置浓度下,Hcy均可诱导HUVECs中MCPIP的表达（P＜0.05）,其中以100 μmol/L Hcy作用24 h时MCPIP表达量最多;100 μmol/l Hcy与细胞作用2h时后MCPIP表达与对照组相比即有明显增高（P＜0.05）,并存在时间依赖效应.结论 Hcy可诱导HUVECs中MCPIP的表达,这可能与它致As的机理有关.

Homocysteine induces monocyte chemoattractant protein-1 induced protein expression in human umbilical vein endothelial cells

Objective To investigate whether homoeysteine （Hcy） induced Monoeyte chemoattractant protein - 1 induced protein （MCPIP） expression in human umbilical vein endothelial cells （HUVECs）. Methods HUVECs were cultured and identified in vitro. 2. HUVECs were treated with different concentrations of Hey （10,50,100, 200 and 500 μmoL/L） for 24 h and were stimulated with Hey （ 100 μmol/L） at differenttimes （ 1,2,4,8,12,24 h）. The MCPIP mRNA and protein expressions were detected by real - time fluorescence quantitative PCR （ Real -time PCR） and western blotting assay, respectively. Results Different concentrations of Hey induced MCPIP expression in HUVECs with the significantly highest MCPIP production 24 h after Hey （100 μmol/L） treatment. Hey （ 100 μmol/L） time - dependently induced HUVECs MCPIP expression from Hey treatment for 2 h （P 〈 0. 05 ）. Conclusion Hey induces MCPIP expression in HUVECs, which might be related to the pathogenesis of ath- erosclerosis.