多囊卵巢综合征患者黄素化颗粒细胞中PDCD5蛋白的表达

目的:了解程序化细胞死亡蛋白5(programmed cell death 5,PDCD5)在多囊卵巢综合征(polysystic ovary syndrome,PCOS)及对照组卵巢颗粒细胞中的表达情况,探讨PDCD5与PCOS的发病关系.方法:选择体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF)中PCOS患者及对照组各30例,收集其颗粒细胞,分别用流式细胞技术、直接免疫荧光以及免疫组化技术检测PDCD5蛋白在颗粒细胞中的表达水平及定位.结果:与对照组相比,PCOS颗粒细胞亚二倍体细胞数明显增加,颗粒细胞中PDCD5的表达明显上调(P<0.05),PDCD5阳性细胞主要表达在胞浆,部分表达在胞核.结论:PCOS患者黄素化颗粒细胞凋亡较对照组增加,提示细胞凋亡可能在PCOS的发病中起一定作用, 凋亡调控蛋白PDCD5可能参与了正常及PCOS卵巢颗粒细胞的凋亡.

Expression of novel apoptosis-related protein PDCD5 in granulosa cells of polysystic ovary syndrome

Objective: To detect the novel apoptosis-related protein PDCD5 expression in granulosa cells of polysystic ovary syndrome(PCOS) and normal ovary, and explore the pathogenesis of PCOS. Methods:The granulosa cells were collected from 30 cases of PCOS and normal ovary in IVF-ET. Expression of PDCD5 was detected by flow cytometry; immunofluorescence and immunohistochemistry. Cell apoptosis was detected by Propidium Iodide (PI) staining. Results: The number of hypodiploidy cells associated with apoptosis in granulosa cells of PCOS was greater than that of the normal control. PDCD5 protein expression in PCOS granulosa cells was significantly higher than that in normal ovary(P<0.05). PDCD5-positive cells were mainly observed in cytoplasm. There was no significant difference between the two groups in terms of age, basal serum FSH concentration, number of ampules of FSH administered. Conclusion: Our data suggest that PDCD5 is an important apoptosis regulating factor in granulosa cells of PCOS and normal ovary, which might be involved in the pathogenesis of PCOS.