砷与铅对人及大鼠红细胞脂质过氧化作用的影响

通过体内体外实验探讨了砷与铅对机体脂质过氧化（ＬＰＯ）的联合作用。用离体人红细胞膜染毒，砷（Ａｓ２Ｏ３）在体系中浓度分别是０，０．４，２．０，１０．０及２０．０ｍｍｏｌ／Ｌ；铅（ＰｂＡｃ２）在体系中浓度分别是０，０．０８，０．４及２．０ｍｍｏｌ／Ｌ；联合染毒采用２×２析因设计，各取其剂量的最低无作用剂量，３７℃孵育６０ｍｉｎ测定ＬＰＯ水平。结果显示，砷与铅对离体人红细胞膜ＬＰＯ的影响具有联合作用，其作用形式为相加作用。为观察砷与铅对整体动物的影响，我们采用２×２析因设计，分别取ＬＤ５０的１／５剂量以灌胃的方式染毒，砷为２．４ｍｇ／ｋｇ，铅为３０ｍｇ／ｋｇ，连续１０天。结果显示，砷与铅对大鼠血浆ＬＰＯ作用不明显，但引起红细胞超氧化物歧化酶（ＳＯＤ）活性的明显抑制，红细胞谷胱苷肽（ＧＳＨ）含量下降；析因分析表明，砷与铅对大鼠红细胞ＳＯＤ活性抑制具有联合作用，联合作用形式为相加作用

Joint Effects of Arsenic and Lead on Lipid Peroxidation of Human and Rat Erythrocytes

The authors observed the joint effects of arsenic and lead on lipid peroxidation(LPO) in vitro and in vivo.In vitro,the authors used the extracted erythrocyte membrane of health adult men and divided them into three exposed groups.The first group was exposed to As_2O_3(0,0.4,2.0 and 10. 0mmol/L).The second group was exposed to PbAc 2 (0,0. 08,0 .4 and 2.0mmol/L).The third group was exposed to As(10mmol/L)+Pb(0 08mmol/L).The results show that there is interaction between As and Pb on LPO of erythrocyte membrane.In vivo,the authors measured LPO levels of rat's plasma,blood glutathione(GSH) and superoxide dismutase(SOD) activity.By using 2×2 factorial design,Wistar rats were gavaged with As_2O_3 2.4mg/(kg·d) and PbAc 2.30mg/(kg·d) as well as both arsenic and lead in water for 10 days.The results show that in the As Pb treated rats,the levels of LPO did not change significantly,the GSH levels and SOD activity decreased significantly.The results of factorial analysis revealed a synergic effect of As Pb on SOD activity.