BCG多克隆抗体在结核性病变组织中的诊断价值

目的 :探讨用卡介苗 (BCG)多克隆抗体检测结核性病变组织中BCG抗原对结核病的诊断价值。方法　收集各种组织不同类型的结核性病变组织标本 175例 ,非结核性病变组织标本 5 4例 ,采用BCG多克隆抗体免疫组化染色标记检测组织中BCG抗原 ,同时与细菌学的抗酸染色作比较。结果　结核组对BCG呈阳性反应者 135例 ,占 77 12 %。阳性染色在朗汉斯巨细胞、上皮样细胞的胞质及干酪样坏死组织呈鲜红色颗粒 ;而抗酸染色阳性仅有 11例。占 6 2 9%。结核病的基本病理类型对BCG反应的阳性率分别是渗出为主型 >坏死为主型 >增殖为主型。结论　采用BCG多克隆抗体的免疫组化标记对结核病诊断的敏感性明显高于抗酸染色 ,有一定的鉴别诊断价值

Diagnostic value of BCG polyclonal antibody in tuberculous

Purpose To study the value of immunohistochemical stain technique in the diagnosis of tuberculous by using BCG polyclonal antibody. Methods One hundred and seventy five cases of tissue samples with various types of tuberculous lesion and 54 cases with non tuberculous lesion were collected. BCG antigen in the tissue was detected by BCG polyclonal antibody by the application of immunohistochemical technique, which was compared with acid fast stain in bacteriology. Results 135 cases (77 12%) in tuberculous group presented positive to BCG. The positive stain demonstrated bright red granules in Langhans giant cells and caseous necrotic tissue. Nevertheless, only 11 cases (6 29%) presented positive acid fast stain. The positive rate to BCG reaction among the basic pathological types of tuberculosis was as follows: exudation type>necrosis type>proliferation type. Conclusions The immunohistochmical technique using BCG polyclonal antibody compared with acid fast stain is far more sensitive for diagnosis of tuberculosis and can be a test for differential diagnosis.