大黄素对Cx32组成的细胞缝隙连接通讯功能的影响 |
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引用本文: | 吕显林,王琴,陈清,陶亮.大黄素对Cx32组成的细胞缝隙连接通讯功能的影响[J].中国现代药物应用,2011,5(10):3-4. |
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作者姓名: | 吕显林 王琴 陈清 陶亮 |
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作者单位: | 1. 东莞市横沥人民医院药剂科,523460 2. 中山大学医学院药理学教研室 3. 中山大学附属第一医院药学部 |
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摘 要: | 目的在转染并稳定表达Cx32的Hela细胞上,观察大黄素对Cx32的GJIC以及Cx32蛋白表达水平的影响。方法采用SRB法检测不同浓度大黄素对Hela细胞的毒性;用细胞接种荧光示踪法("parachute"dye-couplinga ssay)观察不同浓度大黄素对GJIC的影响;用western blot法研究大黄素在影响GJIC功能浓度范围内对Cx32蛋白表达的影响。结果大黄素在0~1μmol/L浓度时对Hela细胞无毒性作用;大黄素(24~600nmol/L)预处理4h,能浓度依赖性地增强GJIC及Cx32蛋白表达量。结论大黄素能够增强Cx32的细胞GJIC;此增强作用可能与其增加Cx32蛋白表达水平有关。
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关 键 词: | 大黄素 缝隙连接蛋白 缝隙连接通讯 |
The effect of Emodin on the function of gap junction composed of Cx32 |
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Institution: | LVXian-lin,WANG Qin,CHENQing,et al.Department ofPharmacy,Hengli People's Hospital ofDongguan city,Dongguan 523460,China |
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Abstract: | Objective To investigate the effect of Emodin on gap junction intercellular communication and the expression of Cx32 protein in Cx32-transfected Hela cells.Methods SRB assay was used to detect the cytotoxicity of emodin on Hela cells.The function of GJIC was determined by"parachute"dye-coupling assay;Western blotwas performed to detectthe expression of Cx32·Results Emodin with concentration of0 ~1μmol/Lhad no cytotoxicity on Hela cells;Pre-treatment the cells with Emodin with concentration of 24 ~600nmol/L for4 hours could increase the intercellular dye transfer and the Cx32 protein expression.Conclusion Emodin could enhance the function of gap junction in Cx32-transfected Hela cells,probably through increasing the Cx32 expression. |
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Keywords: | Emodin Connexin Gap junction intercellular communication |
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