| 多肽研究——Ⅸ.乙型肝炎病毒表面抗原(HBsAg)Pre-S区域肽段的合成和抗原特异性测定 |
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| 引用本文： | 毛峰,王超,程铁明,蔡孟深,张春英,陶其敏. 多肽研究——Ⅸ.乙型肝炎病毒表面抗原(HBsAg)Pre-S区域肽段的合成和抗原特异性测定[J]. 药学学报, 1992, 27(6): 428-433 |
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| 作者姓名： | 毛峰 王超 程铁明 蔡孟深 张春英 陶其敏 |
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| 作者单位： | 北京医科大学药学院,**肝病研究所,北京100083 |
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| 摘 要： | 采用溶液法和固相法,合成了HBsAg Pre-S蛋白区域七个六肽片段。将其与载体蛋白结合。用多克隆抗HBsAg和单克隆抗“α”测定了肽—蛋白结合物的抗原特异性,结果表明P1和P5有较高的抗原特异性。
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| 关 键 词： | 合成多肽 乙型肝炎表面抗原 疫苗 |
| 收稿时间： | 1991-07-09 |
Studies on polypeptides. IX. Synthesis and antigen specificity of pre-S region fragments of hepatitis B surface antigen] |
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| F Mao,C Wang,TM Cheng,MS Cai,CY Zhang,QM Tao. Studies on polypeptides. IX. Synthesis and antigen specificity of pre-S region fragments of hepatitis B surface antigen][J]. Acta pharmaceutica Sinica, 1992, 27(6): 428-433 |
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| Authors: | F Mao C Wang TM Cheng MS Cai CY Zhang QM Tao |
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| Affiliation: | School of Pharmacy, Beijing Medical University. |
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| Abstract: | Seven peptide fragments of HBsAg Pre-S region have been synthesized by liquid phase and solid phase methods. All peptide fragments were linked to protein carrier by carbodiimide and glutaraldehyde methods. The antigen specificity of the peptide--protein conjugates was assayed by anti-HBsAg polyclonal antibodies and anti--"a" monoclonal antibody. P1 and P5 were found to have higher antigen specificity. |
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| Keywords: | HBsAg Vaccine Synthetic polypeptide |
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