| 角质形成细胞对胞内金黄色葡萄球菌抗菌作用研究 |
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| 引用本文： | 刘明岩,耿会珍,蒋献,黄宁,王伯瑶,吴琦.角质形成细胞对胞内金黄色葡萄球菌抗菌作用研究[J].四川生理科学杂志,2010,32(1):10-12. |
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| 作者姓名： | 刘明岩 耿会珍 蒋献 黄宁 王伯瑶 吴琦 |
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| 作者单位： | 1. 四川大学华西基础医学与法医学院感染免疫研究室,四川,成都,610041
2. 四川大学华西医院皮肤科,四川,成都,610041 |
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| 摘 要： | 目的:观察金黄色葡萄球菌在人角质形成细胞株HaCaT中生存的动态变化,了解金黄色葡萄球菌与皮肤角质形成细胞之间的相互关系。方法:用金黄色葡萄球菌标准株ATCC25923侵袭HaCaT细胞,分别于细菌进入细胞后的4、24、48、72h裂解细胞,释放出细胞内的活细菌,用平板菌落计数法计数胞内活菌。结果:金黄色葡萄球菌ATCC25923株在进入HaCaT细胞的24h有一定生长,但实验48h细胞内活菌数量明显减少。蛋白激酶C激活剂PMA和腺苷酸环化酶激活剂FSK可以促进HaCaT细胞清除胞内细菌。结论:皮肤角质形成细胞清除进入细胞内的金黄色葡萄球菌,可能是皮肤天然免疫的一种防御机制,而PMA和FSK增强细胞的抗菌作用,提示角质形成细胞抗菌活性与NADPH氧化酶相关。
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| 关 键 词： | 角质形成细胞 金黄色葡萄球菌 抗菌作用 |
Antibacterial effect of human epidermal keratinocytes against intracellular Staphylococcus aureus |
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| Liu Ming-yan,Geng Hui-zhen,Jiang Xian,Wang Bo-yao,Huang Ning,Wu Qi.Antibacterial effect of human epidermal keratinocytes against intracellular Staphylococcus aureus[J].Sichuan Journal of Physiological Sciences,2010,32(1):10-12. |
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| Authors: | Liu Ming-yan Geng Hui-zhen Jiang Xian Wang Bo-yao Huang Ning Wu Qi |
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| Institution: | Liu Ming-yan1,Geng Hui-zhen1,Jiang Xian2,Wang Bo-yao1,Huang Ning1,Wu Qi1(1.Research Unit of Infection , Immunity,West China Medical Center,Sichuan University,Chengdu 610041,2.Dermatology Department of Huaxi Hospital,Chengdu 610041) |
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| Abstract: | Objective:To further understand the host-pathogen interaction in the human skin,the dynamic survival of Staphylococcus aureus in the human epidermal keratinocytes was analyzed.Methods:The monolayers of human keratinocyte cell line HaCaT were incubated with Staphylococcus aureus ATCC29523 for 2h.After the extracellular bacteria were killed with gentamicin,HaCaT cells were lysed at different time points from 4 hours to 72 huors with Triton X-100 and viable intracellular bacteria were quantified by quantitativ... |
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| Keywords: | Keratinocytes Staphylococcus aureus Antibacterial activity |
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