Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA

When barley protoplasts were inoculated with brome mosaic virus (BMV) RNAs 1 and 2, there was a pronounced synthesis of the 110,000- and 100,000-dalton virally coded proteins. In contrast, there was no detectable synthesis of any viral proteins following inoculation with RNA 3 alone or RNA 4 alone. When RNAs 1 and 2 were recombined with RNA 3 in the inoculum, the profile of proteins synthesized was identical to that following inoculation with similar quantities of unfractionated BMV RNA; i.e., the 35,000-dalton virally coded protein and coat protein were synthesized in addition to the two high-molecular-weight viral polypeptides. RNAs 1 and 2 were shown not to be selectively bound (in preference to RNAs 3 or 4); hence, these data reveal that one or both of these RNAs encode proteins involved in early events of infection, perhaps replication.