mMCP-1对病毒性心肌炎小鼠心肌病变的干预作用

目的研究N端2—8位氨基酸缺失的突变型MCP-1（mutated MCP-1，mMCP-1）对病毒性心肌炎小鼠心肌病变的干预作用。方法CVB3腹腔注射BALB／c小鼠，随机分组：病毒性心肌炎组（A组）、mMCP-1干预组（B组）、空质粒pVAX1对照组（C组）和未感染生理盐水对照组（D组）。比较各组小鼠心脏重量和体重的比值（HW／BW）、心肌组织病理学积分（PS）、心肌组织病毒载量、血清CK—MB水平和心肌浸润的单个核细胞变化。结果与A组相比，B组血清CK—MB水平降低（P〈0．01）、PS减少（P〈0．05），HW／BW和CVB3载量未呈明显改变（P〉0．05）；B组小鼠心肌浸润的单个核细胞减少（P〈0．01），平均下降41、7％；与D组相比，B组CK—MB、PS、HW／BW和CVB3载量均明显升高。而与A组相比，C组CVB3载量、血清CK—MB水平、HW／BW和心肌组织病理学积分均未呈明显改变。结论mMCP-1可缓解病毒性心肌炎中心肌组织病变和心肌损伤，这与单个核细胞浸润减少有关。

Mutated monocyte chemoattractant protein-1(mMCP-1)interferes with the cardiac lesion of viral myocarditis

Objective To investigate the intervention of monocyte chemoattractant protein-1(MCP-1) deleted the 2-8 amino acid in N-terminus(mutated MCP-1,mMCP-1) with the cardiac lesion in viral myocarditis induced by coxsackievirus group B type 3(CVB3).Methods BALB/c mice inoculated intraperitoneally with CVB3 were divided into four groups including viral myocarditis group infected alone with CVB3(group A),intervention group with mMCP-1(goup B),control group with plasma pVAX(group C),and the normal control group(group D) inoculated intraperitoneally with saline was designed.Ratio of mouse heart weight to body weight(HW/BW),myocardial tissue pathological score(PS) of cardiac tissue,the level of CK-MB in serum and CVB3 loading of myocardial tissue were analyzed.Results Compared with group A,the level of creatine kinase-MB fraction(CK-MB) in group B decreased(P<0.01) and PS also reduced(P<0.05),but HW/BW and CVB3 loading in myocardial tissue did not changed greatly(P>0.05).And there was much difference between group B and group D in the level of CK-MB,PS and CVB3 loading of myocardial tissue.Compared with Group A,group C's CVB3 loading in myocardial tissue,CK-MB level in serum,HW/BW and PS all did not alter markedly(P>0.05).Conclusion mMCP-1 could alleviate the cardiac lesion and cardiac injury of viral myocarditis,which is associated with improvement of the infiltration of mononuclear cells.