| Jagged1真核表达质粒构建及其对VSMCs增殖凋亡的影响 |
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| 引用本文： | 钱德慧,武晓静,姜洪,匡春燕,王逵,宋明宝,黄岚.Jagged1真核表达质粒构建及其对VSMCs增殖凋亡的影响[J].第三军医大学学报,2011,33(11):1095-1098. |
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| 作者姓名： | 钱德慧 武晓静 姜洪 匡春燕 王逵 宋明宝 黄岚 |
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| 作者单位： | 第三军医大学新桥医院心血管内科,全军心血管病研究所,重庆,400037 |
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| 摘 要： | 目的构建大鼠Jagged1真核表达质粒,分析Jagged1过表达对血管平滑肌细胞(vascular smooth musclecells,VSMCs)增殖和凋亡的影响。方法从带Jagged1全长基因质粒pBOS-SN3T中酶切获得目的基因,用载体pCMV-IRES-GFP构建重组质粒,转染至VSMCs。Western blot检测Jagged1蛋白表达变化,3 H]-TdR掺入法检测细胞增殖,流式细胞仪(FACS)检测细胞凋亡。结果成功构建大鼠Jagged1真核表达载体并转染至VSMCs,经G418筛选后转染率可达90%以上,转染上调了VSMCs的Jagged1蛋白水平约6倍。转染p-rJagged1后VSMCs的增殖能力明显降低(10 265±1 004)cpm/孔vs(17 584±1 587)cpm/孔,P<0.01],而凋亡明显增高总凋亡率:(32.35±2.86)%vs(15.24±1.27)%;早期凋亡率:(22.47±2.54)%vs(10.22±0.98)%;P<0.01]。结论成功构建大鼠Jagged1真核表达质粒,过表达Jagged1可抑制VSMCs增殖并促进其凋亡。
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| 关 键 词： | 平滑肌细胞 细胞增殖 细胞凋亡 Notch通路 |
Construction of eukaryotic vector expressing Jagged1 gene and its effect on proliferation and apoptosis of vascular smooth muscle cells |
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| Qian Dehui,Wu Xiaojing,Jiang Hong,Kuang Chunyan,Wang Kui,Song Mingbao,Huang Lan.Construction of eukaryotic vector expressing Jagged1 gene and its effect on proliferation and apoptosis of vascular smooth muscle cells[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(11):1095-1098. |
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| Authors: | Qian Dehui Wu Xiaojing Jiang Hong Kuang Chunyan Wang Kui Song Mingbao Huang Lan |
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| Institution: | Qian Dehui,Wu Xiaojing,Jiang Hong,Kuang Chunyan,Wang Kui,Song Mingbao,Huang Lan(Institute of Cardiovascular Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing,400037,China) |
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| Abstract: | Objective To construct a recombinant eukaryotic vector expressing rat Jagged1,and to explore the effect of Jagged1 over-expression on the proliferation and apoptosis of vascular smooth muscle cells(VSMCs).Methods The full-length fragment of Jagged1 gene was obtained from the plasmid pBOS-SN3T(encoding rat Jagged1) by digestion.A eukaryotic vector expressing rat Jagged1(p-rJagged1) was constructed with the aid of the plasmid pCMV-IRES-GFP,and then the vectors were transfected into VSMCs.The expression of Jag... |
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| Keywords: | smooth muscle cells cell proliferation cell apoptosis Notch pathway |
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