Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method: a multicenter investigation |
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| Authors: | Melissa K. Deck Erica S. Anderson Rebecca J. Buckner Georgia Colasante Thomas E. Davis James M. Coull Benjamin Crystal Phyllis Della Latta Martin Fuchs Deanna Fuller Will Harris Kevin Hazen Lisa L. Klimas Daniel Lindao Michelle C. Meltzer Margie Morgan Janeen Shepard Sharon Stevens Fann Wu Mark J. Fiandaca |
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| Affiliation: | 1. AdvanDx Inc., Woburn, MA;2. Indiana University School of Medicine, Indianapolis, IN;3. Health Network Laboratories, Allentown, PA;4. Columbia University Medical Center, NewYork-Presbyterian Medical Center, New York, NY;5. Duke University, Durham, NC;6. Cedars-Sinai Medical Center, Los Angeles, CA |
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| Abstract: | The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which uses a simplified, faster assay procedure. The test uses fluorescently labeled PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other Enterococcus spp. by the color of the cellular fluorescence. Three hundred fifty-six routine blood culture samples were tested; only 2 discordant results were recorded. The sensitivities for detection of Enterococcus faecalis and non-faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, and the combined specificity of the assay was 100%. The combined positive and negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), respectively. |
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| Keywords: | QuickFISH septicemia PNA Enterococcus |
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