Identification, cDNA cloning and heterologous expression of a hyaluronidase from the tarantula Brachypelma vagans venom |
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| Authors: | Herlinda Clement Alejandro Olvera,Mabel Rodrí guez Fernando Zamudio,Laura A. Palomares Lourival D. Possani,George V. Odell |
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| Affiliation: | Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor. 62250, Mexico |
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| Abstract: | Hyaluronidases (Hyal) present in the venom of poisonous animals have been considered as “spreading factors” that facilitate a fast penetration of the venom in the prey. We have found that hyaluronidase from the tarantula Brachypelma vagans venom (BvHyal) displays a substrate-specific Hyal activity against hyaluronan. By using a combined strategy based on peptide sequencing and RT-PCR, we have cloned a BvHyal cDNA. Active recombinant BvHyal was efficiently expressed in a baculovirus system in insect cell. |
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| Keywords: | Tarantula Venom Brachypelma vagans Hyaluronidase Baculovirus Recombinant protein |
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