MIF在小鼠同种皮肤移植排斥过程中的表达

目的：通过对巨噬细胞游走抑制因子(MIF)在小鼠同种皮肤移植排斥前后脾组织和移植皮片中的相对表达量分析，探讨MIF表达与小鼠同种皮肤移植排斥的关系。方法：利用小鼠同种皮肤移植模型，对皮肤移植后不同时间的小鼠脾细胞和移植皮片进行RNA提取及逆转录聚合酶链反应(RT-PCR)，以β肌动蛋白(β-actin)作为对照，比较移植前后MIF的相对表达量。结果：移植后的移植皮片中MIF的相对表达量有明显变化，移植后第1、7、14天，逐渐升高，在排斥高峰期第14天达到最高，第21天皮片完全排斥后下降，但仍比移植前水平高。在移植过程中，脾细胞MIF相对表达量无明显变化。结论：在移植排斥过程中，移植物局部MIFmRNA表达增强，提示MIF可能参与了小鼠同种皮肤移植的局部排斥反应。

Expression of MIF mRNA in murine skin allograft rejection

Objective: To observe the expression of MIF mRNA in murine skin allograft rejection. Methods: Total RNA was exrracted from the skin graft and spleen tissue in murine allograft models at different times before and after the transplantation, and the relative expression amount of MIF in the product of RT-PCR for each sample was compared. Results: The relative expression amount of MIF in the skin graft markedly changed after the transplantation, that is, there is a gradual increase on day 1,7,14 post-transplantation and a peak at the top period of the rejection, and then it decreased after the complete rejection. The relative expression amount in the spleen cells did not change obviously. Conclusion: The expression of MIF mRNA in the graft was enhanced during the graft rejection.