自动血液洗涤仪制备冰冻保存红细胞的方法评价

目的应用215型血液洗涤仪制备低温(-70℃)冰冻保存甘油化红细胞,冻存一定期限后再洗涤红细胞去甘油,检测冰冻和洗涤红细胞的质量指标,并与国家标准比较,评价制备方法和效果。方法随机取4℃保存10d内的红细胞悬液20袋,用215型血液洗涤仪制备甘油化红细胞,-70℃低温冰箱保存90d,然后取出于37℃水浴快速融化,用血液洗涤仪洗涤去甘油。结果冰冻洗涤去甘油红细胞平均红细胞回收率(83.68±4.21)%,游离血红蛋白浓度(0.34±0.12)g/L,体外溶血试验(31.56±14.66)%,残留白细胞(0.58±0.16)%,残留血小板0%,甘油残余量(6.66±1.19)g/L,细菌培养试验阴性,满足国标《全血及成分血质量标准》的要求。结论 215型血液洗涤机可自动制备低温冰冻长期保存红细胞和并洗涤红细胞去甘油,操作简单,快速并标准化,所获洗涤红细胞质量符合国家标准,值得推广应用。

EVALUATION OF THE METHOD TO PROCESS THE GLYCEROLIZED FROZEN RED BLOOD CELLS(RBCS) WITH HAEMONETICS ACP 215 MACHINE

ObjectiveTo evaluate the method to process the glycerolized frozen red blood cells（RBCs） and thawing,washing RBCs for deglycerolization with Haemonetics ACP 215 machine.Methods Glycerolized 4℃ stored 10 days RBCs and frozen at-70℃ for 90 days,then thawing at 37℃ and washing RBCs for deglycerolization with Haemonetics ACP 215 machine.Results For the post-washing storage RBCs,the RBCs recovery rate is （83.68±4.21）%,free hamoglubin（Hb） is （0.34±0.1 2）g/L,haemolysis test is （31.56±14.66）%,white cells remain is （0.58±0.16）%,platelets remain is 0%,glycerol remain is （6.66±1.19）g/L,bacteria test is negative.All the quality parameters can meet the National Requirements for Blood Products.Conclusion Using Haemonetics ACP 215 machine to process the glycerolized frozen RBCs and washing RBCs for deglycerolization for RBCs long-time storage is an effective and applicative method.The RBCs product can meet the quality requirements.