烟雾对组蛋白去乙酰化酶2在COPD大鼠肺组织中表达的影响

 摘要 目的 比较接触烟雾不同时间后慢性阻塞性肺疾病(chronic obstructive pulmonary disease, COPD)大鼠肺组织之间及与正常大鼠肺组织中组蛋白去乙酰化酶2（histone deacetylase 2，HDAC2）表达的差异，探讨烟雾对组蛋白去乙酰化酶2在COPD大鼠肺组织中表达的影响。 方法 在自制密闭有机玻璃箱内让大鼠吸入香烟烟雾，实验组根据烟熏和COPD形成时间不同分为4组，每组10只大鼠；对照组为正常大鼠10只。免疫组织化学法和原位杂交法分别测定HDAC2的蛋白和mRNA在肺组织中的表达。 结果 实验各组HDAC2蛋白质和mRNA的A值均低于对照组（p<0.05），实验组各组间比较p值也均小于0.05，差异有统计学意义。结论 HDAC2在实验组COPD大鼠肺组织中的表达低于正常对照组，而且随着接触烟雾时间延长呈下降趋势。

The Effect of Smoking On Expressing of Histone Deacetylase 2 in the Lung Tissues of COPD Rats

Abstract：Objective To investigate the relationship of histone deacetylase 2 (HDAC2) and the inflammation of chronic obstructive pulmonary disease (COPD) by detecting its expression in lung tissues of COPD rats and normal rats. Methods：We made the COPD rat model by cigarette smoking. According to the time of COPD formation, we divided the rats into four groups, there were 10 rats in every group including the control group. The expression of HDAC2 protein and mRNA were detected by immunohistochemical and in situ hybridization assays. Results：The A values of HDAC2 protein and mRNA in COPD rats lung tissues were lower than that of normal rats (p<0.05). And between the COPD groups the differences were significant (p< 0.05). Conclusion：The expression of HDAC2 in COPD rats was lower than that of normal rats, and depresseded with the progression of COPD.