氯喹对体外培养星形胶质细胞激活的抑制作用

  目的 研究氯喹对体外培养大鼠海马星形胶质细胞激活的抑制作用，为癫痫的治疗提供实验依据。方法 分离新生SD大鼠海马，体外培养星形胶质细胞，经纯化鉴定后分组：a 对照组；b 戊四氮（PTZ）组；c 氯喹干预组（25mg、50mg、75mg/L），经相应处理后，分别运用MTT法、免疫荧光、western-blot测定星形胶质细胞数量及活性、星形胶质纤维酸性蛋白(GFAP)及CyclinD1的表达量，并分析比较各组星形胶质细胞激活情况。结果 与b组比较，MTT法显示氯喹阻滞了PTZ激活的星形胶质细胞的增殖（P<0.05）；免疫荧光结果显示，氯喹可抑制PTZ激活星形胶质细胞异常增加的GFAP的表达；western-blot结果显示氯喹可抑制PTZ激活星形胶质细胞的CyclinD1的表达量（P<0.05）；与a组比较，3种结果均显示75mg/L氯喹对体外培养星形胶质细胞激活的抑制作用较强，并可维持其在正常范围。结论 氯喹具有抑制PTZ激活体外培养星形胶质细胞的作用，其可能通过抑制星形胶质细胞的增殖来发挥抗癫痫作用。

Inhibitory Effects of Chloroquine on Astrocyte Activation in Vitro

Objective To investigate the effects of Chliroquine on Pentylenetetrazole(PTZ)- activitied hippocampal astrocyte of rats. Methods Neonatal rat (24h) hippocampal astrocytes were obtained and divided into contron group, PTZ-activitied group and Chliroquine groups with Chliroquine treatment at 25, 50, 75mg/ L. The cell proliferation, expression of glial fibrillary acidic protein (GFAP) and CyclinD1 after the treatments were detected with MTT assay, immunofluorescence cytochemistry and western bloting respectively. Results In comparison with PTZ-activition group, Chliroquine treatment resulted in significant inhibition of the cell proliferation, GFAP and CyclinD1 in the PTZ-activated astrocytes (P<0.05). Chliroquine at 75mg/L showed the strongest inhibitory effects against astrocyte activation and maintained nearly normol level of astrocyte activation in comparison with the control group. Conclusion Chliroquine could inhibited the activation of astrocytes which activitied by PTZ. So Chliroquine may be used to treat epilepsy.