| 重组腺相关病毒介导红荧光蛋白在骨髓间充质干细胞的表达 |
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| 引用本文： | 刘晓红,龚德军,袁扬,黄盛东.重组腺相关病毒介导红荧光蛋白在骨髓间充质干细胞的表达[J].实用医药杂志(山东),2006,23(3):332-334. |
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| 作者姓名： | 刘晓红 龚德军 袁扬 黄盛东 |
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| 作者单位： | 第二军医大学附属长海医院胸心外科,上海200433 |
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| 摘 要： | 目的构建表达红荧光(DsRed)重组腺相关病毒,研究腺相关病毒对骨髓间充质干细胞(BMSCs)的转染效率。方法DsRed目的基因经酶切插入载体质粒pAAV-MCS,构建重组质粒pAAV-DsRed。磷酸钙法转染AAV-293细胞获取重组腺相关病毒。将AAV-DsRed体外转染原代培养的兔BMSCs,分析其转染效率。结果成功构建重组质粒pAAV-DsRed,获得腺相关病毒AAV-DsRed,并感染兔BMSCs。结论腺相关病毒能够转染BMSCs并表达外源基因,具有应用于临床治疗的潜力。
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| 关 键 词： | 红荧光蛋白 腺相关病毒 骨髓间充质干细胞 |
| 修稿时间： | 2006年1月13日 |
Expression of red fluorescent protein mediated by recombinant adeno-associated virus in bone marrow mesenchymal stem cells |
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| LIU Xiao-hong,GONG De-jun,YUAN Yang,et al..Expression of red fluorescent protein mediated by recombinant adeno-associated virus in bone marrow mesenchymal stem cells[J].Practical Journal of Medicine & Pharmacy,2006,23(3):332-334. |
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| Authors: | LIU Xiao-hong GONG De-jun YUAN Yang |
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| Institution: | LIU Xiao-hong,GONG De-jun,YUAN Yang,et al. Department of Cardiothoracic Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | Objective To construct the recombinant adeno-associated virus expressing red fluorescent(DsRed)protein and investigate its transfection efficiency in vitro.Method The recombinant plasmid pAAV-DsRed was constructed by inserting the DsRed gene into the pAAV-MCS and generating the primary recombinant AAV by calcium phosphate-based technology. The transfection efficiency were evaluated by transfecting AAV-DsRed into primary rabbit BMSCs.The direction of the insertion was confirmed by PCR analysis. Results The recombinant plasmid pAAV-DsRed has been constructed successfully, and AAV-DsRed obtained;the primary BMSCs could be transfected efficiently with AAV-DsRed. Conclusion The recombinant Adeno-associated virus was constructed succeesfuly,it can transfect BMSCs and express exogenous gene, thus possess the potential for clinical application. |
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| Keywords: | Red fluorescent protein Recombinant adeno-associated virus Bone marrow mesenchymal stem cells |
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