Determination of serum and organ malondialdehyde (MDA) concentration, a lipid peroxidation index, in Trypanosoma brucei-infected rats |
| |
Authors: | J I Eze B M Anene C C Chukwu |
| |
Institution: | (1) Department of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria |
| |
Abstract: | Lipid peroxidation was assessed in the sera and various organs of rats experimentally infected with Trypanosoma brucei. Thirty-six adult albino rats divided into 2 groups of eighteen rats each were used in this study. In experiment one, a group
of 18 rats were used and they were divided into three groups (A, B and C) of six rats each. Groups B and C rats were infected
with 1.54 × 105 trypanosomes per rat intraperitoneally, whereas group A served as uninfected control. The rats were bled on day 0 and subsequently
at 7-day intervals for packed cell volume (PCV), sera peroxidation index and parasitaemia. Also, temperature and weight were
taken on day 0 and subsequently at 7-day intervals. In experiment 2, 18 rats were also used. Six rats each were sacrificed
on days 0, 14 and 28-postinfection. Five rats each were sacrificed on day 14 and day 28 post-infection (PI) from group B,
and their organs were promptly collected and washed with normal saline and used for organ malondialdehyde (MDA) concentration.
The infection led to an increase in lipid peroxidation index (MDA concentration) of sera samples. The serum MDA concentration
of the infected rat group was significantly (p < 0.01) higher than in the uninfected group on days 21 and 28 PI. The increase was however reversed by diminazene aceturate
(Berenil; Hoechst, Ireland) treatment at the dosage of 7 mg/kg body weight administered on day 14 PI. The organ lipid peroxidation
index also increased significantly (p < 0.05) in the eye, lung and spleen. However, there was no significant (p > 0.05) increase of lipid peroxidation index in the kidney, heart, liver, testes and brain. Also, the mean weekly MDA concentration
increased as the disease progressed, the mean weekly temperature and parasitaemia also increased, but the reverse was the
case with the mean weekly body weight and PCV which declined as the disease progressed. The findings are indication that oxidative
stress plays an important role in the pathology of trypanosomosis. |
| |
Keywords: | Malondialdehyde Organs Rats Sera Trypanosoma brucei brucei |
本文献已被 SpringerLink 等数据库收录! |
|