预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响

目的：探讨预防性针刺不同疗程足三里穴对应激大鼠胃粘膜损伤的保护作用的内在机制。方法：雄性SD大鼠40只，随机分为空白对照组、应激组、电针1d组、电针3d组、电针5d组，每组8只。利用激光多普勒血流仪测定胃粘膜血流量，用放免分析法测定各组大鼠血浆ET，PGE2的变化，用生化法测定NO的含量，并计算各组溃疡指数（UI）。结果：应激组与空白对照组比较，GMBF明显下降，UI明显上升（P〈0．01），而血浆PGE2、NO水平下降。ET水平上升（P〈0．05）。电针1d组与应激组比较，GMBF上升，UI下降（P〈0．01）；血浆PGE2、NO水平上升（P〈0．01或P〈0．05），ET水平下降（P〈0．05）。电针5d组与电针1d组比较，GMBF、POE2、NO上升更职显，而UI则职显下降（P〈0．05）。电针3d组与电针1d组、电针5d组指标比较，无明显统计学意义。电针1、3、5d组之间指标ET血浆水平无明显统计学意义。结论：预防性针刺足三里穴对应激大鼠胃粘膜损伤具有保护作用，且具有时间一剂量效应。其机制可能与词节机体内血浆ET、NO、PGE2的水平有关。

Effects of preventive electroacupuncture at Zusanli point on gastric mucosa in stress rats

Objective: To study the effects of preventive electroacupuncture at Zusanli point for different times on gastric mucosa in stress rats. Methods: 40 male SD rats were divided randomly into 5 groups(each group had 8 rats) including control group, cold stress group and 3 electroacupunture groups in which the animals were acupunctured 30 min at Zusanli point with electroacupuncture a day for 1, 3 or 5 days before challenged by cold. Their gastric mucosal blood flow(GMBF) was measured by laser Doppler flowmeter, the level of plasma endothelin(ET), prostaglandin E2(PGE2) were examined by radioactive -immunoassay and nitric oxide(NO) by biochemical method. The extent of gastric mucosal damage was evaluated according to ulcer index(UI). Results: In the stress group, the mean GMBF decreased, UI increased greatly(P<0.01), the level of PGE2 and NO decreased but ET increased(P<0.05), as compared with those of the control group. Compared with stress group, GMBF increased and UI decreased (P<0.01) in the electroacupuncture 1 day group. In the electroacupuncture 5 day group, GMBF, PGE2 and NO increased, but UI decreased greatly (P<0.05), compared with those of eletroacupuncture 1 day group except of ET. Conclusion: The treatment of preventive electroacupuncture at Zusanli point can protect gastric mucosa in stress rats. This protective function is of time -dose dependenc and may be relative to the changes of level of plasma ET, NO and PGE2.