人结直肠癌组织中EGFR/Grb2/p-mTOR/VEGF的表达及意义

目的:观察人结直肠癌组织磷酸化哺乳动物雷帕霉素靶蛋白(phosphorylated mammalian target of rapamycin,p-mTOR)、表皮生长因子受体(EGFR)、生长因子受体结合蛋白2 (Grb2)和血管内皮生长因子(VEGF)等的表达,并探讨其可能的临床意义。方法:构建含有185例结直肠癌标本的组织芯片,免疫组化法检测结直肠癌组织及癌旁组织中EGFR、Grb2、p-mTOR和VEGF的表达,并分析不同结直肠癌临床病理学特征下(如年龄、性别、浸润深度、淋巴转移、临床分期和分化程度)各自的表达情况,探讨可能的临床意义。结果:EGFR、Grb2、p-mTOR和VEGF在结直肠癌旁组织中呈少量表达或不表达,在结直肠癌组织中的表达率分别为21.1%、44.9%、42.2%和54.1%,明显高于癌旁组织(P<0.05)。结直肠癌患者不同性别、年龄、肿瘤分化程度下EGFR、Grb2、p-mTOR和VEGF表达率无统计学差异；不同浸润深度和临床分期下EGFR的表达率有统计学差异(P<0.05)；不同浸润深度、淋巴转移和临床分期下p-mTOR、VEGF表达率均有统计学差异(P<0.05)。结直肠癌组织EGFR/Grb2/p-mTOR/VEGF蛋白两两间均有一定的相关性(r=0.245~0.567,P<0.05)。结论:EGFR、Grb2、p-mTOR和VEGF表达与结直肠癌的发生、发展相关,值得进一步研究以作为结直肠癌肿瘤靶向治疗新的作用靶点。

Expression of EGFR,Grb2,p-mTOR and VEGF in human colorectal cancer tissues

Aim: To detect the expression of EGFR, Grb2, p-mTOR and VEGF in colorectal cancer and to explore their roles in the carcinogenesis of colorectal cancer. Methods: The expression of EGFR, Grb2, p-mTOR and VEGF in 185 the colorectal cancer specimens and the corresponding adjacent tissues were evaluated by tissue microarray and immunohistochemistry, and the relationship between the expression and the age, sex, invasion depth, lymph metastasis, clinical stage and differentiation degree was analyzed. Results: Diffused and hardly any expression of EGFR, Grb2, p-mTOR and VEGF were found in the adjacent-cancer tissues. The expreesion of EGFR, Grb2, p-mTOR and VEGF was obviously stronger in colorectal cancer tissues compared with that in the adjacent cancer tissues. The over expression rates of EGFR, Grb2, p-mTOR and VEGF in colorectal caner were 21.1%, 44.9%, 42.2% and 54.1%, respectively. Over-expression of EGFR was significantly associated with invasion depth and clinical stage. Over-expression of p-mTOR and VEGF was significantly associated with lymph metastasis, invasion depth and clinical stage. There was no significant association between the Grb2 over-expression and those clinicopathological characteristics. Significant relationship was seen in every two proteins. Conclusion: As EGFR, Grb2, p-mTOR and VEGF serves as signaling pathway related proteins, their expression is closely associated with the malignant phenotype of colorectal cancer. And it is indicated that they may be involved in the development and progress of colorectal cancer and may serve as some targets for molecular target therapy for colorectal cancers.