丝瓜提取物对小鼠巨噬细胞功能的影响

目的　探讨丝瓜的果、叶、藤提取物对小鼠巨噬细胞功能的影响。方法　将 50只小鼠随机等分为正常对照组、阳性对照组、实验Ⅰ组、Ⅱ组和Ⅲ组。正常对照组用生理盐水灌胃 ,实验Ⅰ、Ⅱ、Ⅲ组分别用丝瓜的果、叶和藤提取物灌胃 ,阳性对照组用胸腺肽灌胃 ;10d后检测小鼠腹腔巨噬细胞内酸性磷酸酶活性及巨噬细胞吞噬功能的变化。结果　实验组吞噬功能强度由高到低依次为实验Ⅲ组、实验Ⅰ组和实验Ⅱ组 ,但 3组比较无统计学差异 (P >0 .0 5)。各实验组与正常对照组相比 ,小鼠腹腔巨噬细胞内酸性磷酸酶活性和巨噬细胞吞噬功能均有不同程度增强 (P <0 .0 5) ,与阳性对照组相比无显著差异 (P >0 .0 5)。结论　丝瓜不同部位提取物对小鼠巨噬细胞功能均有增强作用

Effects of Luffa-extract on the macrophage function in mice

Objective To observe the effects of Luffa-extrac t from fruit,leaf and vine on the macrophage function in mice. Methods Fifty mice were randomly divided into five groups: the normal control group, t hree experimental groups and the positive control group. The mice in the normal control groups were perfused with saline; and those in the experimental group we re performed with Luffa-extract derived from fruit,leaf and vine respective ly; and those in the positive control group were perfused with thymopeptide. The stu dy was carried out 10 days later by determining the changes of acid phosphatase (ACP) activity in peritoneal macrophage (PMΦ) and the engulfing function of PM Φ.Results Compared to the control group, the ACP activity and the engulfing function of PMΦ increased in the experimental group,which in orde r of action,group Ⅲ,group Ⅱand group Ⅰ.There was no significant difference c ompared to the positive control group. Conclusion Luffa-ex tract plays a very important role in enhancing macrophage function in mice.