| 微量淋巴细胞毒试验及其影响因素的探讨 |
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| 引用本文： | 徐沈育,宋芳吉,李惠刚,苏霄汉,刘子勇,周亚芬. 微量淋巴细胞毒试验及其影响因素的探讨[J]. 中国医科大学学报, 1984, 0(5) |
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| 作者姓名： | 徐沈育 宋芳吉 李惠刚 苏霄汉 刘子勇 周亚芬 |
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| 作者单位： | 中国医科大学附属第一医院临床免疫研究室(徐沈育，宋芳吉，李惠刚，苏霄汉，刘子勇)，中国医科大学附属第一医院临床免疫研究室(周亚芬) |
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| 摘 要： | 本文初步探讨了T、B微量淋巴细胞毒试验中的影响因素,证明了一般甲醛(pH6.4～6.7)可以用于T淋巴细胞毒试验、并且染色时间在2分钟到5分钟之间对试验结果影响不明显。在B淋巴细胞毒试验中,使用酸性(pH6.4～6.7)和中性(pH7.0～7.2)甲醛固定试验结果差异明显;染色时间须控制在2分钟。
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| 关 键 词： | 淋巴细胞毒 淋巴细胞 |
STUDY ON MICROLYMPHOCYTOTOXITY TEST AND THEIR INFLUENCE FACTORS |
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| Xu Shenyu et al Dept of Dermatology ard Immunology Lab. First Affiliated Hospital. STUDY ON MICROLYMPHOCYTOTOXITY TEST AND THEIR INFLUENCE FACTORS[J]. Journal of China Medical University, 1984, 0(5) |
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| Authors: | Xu Shenyu et al Dept of Dermatology ard Immunology Lab. First Affiliated Hospital |
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| Affiliation: | Xu Shenyu et al Dept of Dermatology ard Immunology Lab. First Affiliated Hospital |
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| Abstract: | Through 1440 pairs of parallel (xpriments the authrs studied various influences (staining time, different pH of formaldehyde)in the B and T microlymphocytotoxicity test. The experimental method used here was stable.These studies showed that the acidity of f(?)r(?)dchyde could be used and the stainirg time between 2 to 5 minutes made no difference for T microlymphocytotoxicity test. In the B microlymphocytotoxicity test, reutral and formaldehyde were rather different and two minute staining time was essential. |
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| Keywords: | Lymphocytotoxiciiy. Lymphocyte |
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