四环素—哌嗪雌酚酮对小鼠骺板I型胶原表达的影响

目的：研究四环素－哌嗪雌酚酮（XW630）对胚胎小鼠骺板I型胶原的表达的影响。方法：采用胚胎小鼠体外培养模型，通过免疫组织化学手段，观察XW630对I型胶原蛋白表达水平的影响。结果：当培养基中XW630浓度为10^-7mol/L和10^-8mol/L时，各区I型胶原蛋白免疫组织化学染色阳性细胞面积与对照组相比均显著增加；与同浓度的雌酚酮组相比，106-7mol/L XW630组增殖区和肥大区阳性细胞面积增加。当浓度降至10^-9mol/L，只有肥大区阳性细胞面积增加。随浓度升高，各区阳性细胞面积呈增加趋势。结论：在体外培养条件下，XW630剂量依赖地上调小鼠骺板静止区、增殖区和肥大区I型胶原蛋白的表达水平。

Effects of XW630 on the expression of type I collagen protein in the epiphyseal plates of mice]

OBJECTIVE: To identify the effect of XW630 on the expression of type I collagen in the epiphyseal plates of mice. METHODS: The immunohistochemical method was used to measure the change of type I collagen protein after being treated with XW630. RESULTS: With 10(-7) mol/L or 10(-7) mol/L of XW630, the area of positive stain cells in every zone increased significantly compared to the control group. A more significant increase of the area of positive cells in the proliferative zone and hypertrophic zone was observed in the XW630 group than in the estrone group at a concentration of 10(-7) mol/L. When the XW630 was decreased to 10(-9) mol/L, the increase of positive cells was only found in the hypertrophic zone. The area of positive cells increased with the concentration of XW630. CONCLUSION: The XW630 upregulates the dose-dependent expression of type I collagen protein in the resting zone, proliferative zone, and hypertrophic zone in the epiphyseal plates of mice in vitro.