肝病患者肝组织中HCV抗原检测及意义

应用免疫组织化学方法对一组肝病患者石蜡包埋肝组织中丙肝毒抗原进行了检测，２５２分肝组织中，采用多克隆抗体ＨＣＶ检出ＨＣＡｇ阳性２５份（９．９２％）采用单克隆抗ＨＣＶ－ＮＳ３检出ＨＣＶ－ＮＳ３蛋白阳性１９份（７．５４％），１９份ＨＣＶ－ＮＳ３阳性肝组织均同时表现ＨＣＡｇ阳性，二者在肝细胞分布状况相似，另有６例肝组织中检出ＨＣＡｇ阳性，阳性细胞周围多无坏死灶和炎性，不同病理类型肝炎患者肝组织中丙肝病毒

DETECTION AND CLINICAL SIGNIFICANCE OF HEPATITISC VIRUS ANTIGEN IN LIVER TISSUESOF PATIENTS WITH LIVER DISEASE

epatitis C virus antigens in paraffin-embeded liver tissues of patients with liver disease were detected byimmunohistochemistrv method to study its expression and clinical significance. HCAg and HCV-NS3 protein weredetected by using polyclonal anti-HCV and monoclonal anti-HCV-NS,and the positive rate were9.92%(25/252)and 7.54%(19/252),respectively.All of the nineteen HCV-NS3 positive tissues were also positive for HCAg.The distribution of them in liver cells was similar.Another six samples were HCAg positive but HCV-NS3negative. There were no obvious necrosis and inflammation aggregaeion around the positive cells in most of thesections.Significant difference of the positive rate of HCV antigen in liver tissues was found among differentmodels of serum HCV markers but not among different pathologic type of hepatitis.No HCV antigens weredetected in the liver of patient with anti-HCV positive only.Still,the positive rate was much lower in the liverof patient with both serum anti-HCV and HCV RNA positive then that of patient with serum HCV RNA positiveonly. We considered that detection of HCV antigen in liver tissue using immunohistochemistry method supplysome help for understanding the pathogenesis of HCV.