| 胰高血糖素样肽1受体激动的高通量筛选及作用机制研究 |
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| 引用本文： | 殷菲,邓小红,景佳佳,郑旭煦,刘建辉. 胰高血糖素样肽1受体激动的高通量筛选及作用机制研究[J]. 中国药学杂志, 2007, 42(1): 24-27 |
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| 作者姓名： | 殷菲 邓小红 景佳佳 郑旭煦 刘建辉 |
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| 作者单位： | 1. 重庆工商大学,药物化学与化学生物学研究中心,重庆,400067;重庆工商大学,环境与生物工程学院,重庆,400067
2. 重庆工商大学,药物化学与化学生物学研究中心,重庆,400067 |
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| 基金项目： | 国家自然科学基金;重庆市科委重点基础项目 |
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| 摘 要： | 目的筛选胰高血糖素样肽1(GLP-1)受体激动剂,并进行初步的作用机制研究。方法建立稳定的GLP-1受体激动剂高通量筛选模型,从天然药物中筛选活性成分;在PC12细胞中验证GLP-1受体激动剂的功能及作用机制。结果经过筛选、鉴定,京尼平苷是一种新的GLP-1受体激动剂,能够通过MAPK信号通路诱导PC12细胞分化,对抗过氧化氢诱导的PC12细胞氧化损伤,提高神经细胞活力。结论京尼平苷具有与GLP-1受体激动剂类似的神经营养和神经保护功能。
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| 关 键 词： | 胰高血糖素样肽1 激动剂 京尼平苷 |
| 文章编号： | 1001-2494（2007）01-0024-04 |
| 收稿时间： | 2006-11-12; |
| 修稿时间： | 2006-11-12 |
Screening of Agonist for Glucagon-Like Peptide-1 Receptor and Its Effect on PC12 Cells |
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| YIN Fei, DENG Xiao-hong, JING Jia-jia, ZHENG Xu-xu, LIU Jian-hui. Screening of Agonist for Glucagon-Like Peptide-1 Receptor and Its Effect on PC12 Cells[J]. Chinese Pharmaceutical Journal, 2007, 42(1): 24-27 |
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| Authors: | YIN Fei DENG Xiao-hong JING Jia-jia ZHENG Xu-xu LIU Jian-hui |
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| Affiliation: | a.Research Center of Pharmaceutical Chemistry & Chemical Biology，b.College of Enviroment and Bioengineering，Chongqing Technology and Business University，Chongqing 400067，China |
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| Abstract: | OBJECTIVE To screen the agonist for glucagon-like peptide-1 receptor,and evaluate its neuronal activity. METHODS Natural products were collected to screen in the stable cell line of GLP-1 receptor ,and the active component was identified in the PC12 cells. RESULTS Geniposide was a novel agonist for GLP-1 receptor,which induced the neuronal differentiation of PC12 cells via mitogen-activated protein kinase pathway,and prevented PC12 cells from oxidative damage induced by hydrogen peroxide. CONCLUSION Geniposide has the similar function as the agonist for GLP-1 receptor,including neurotrophic and neuroprotective effects. |
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| Keywords: | glucagon-like peptide-l agonist geniposide |
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