基于信息熵赋权法的正交试验优化七味蟾参方提取工艺研究

目的:优化七味蟾参方的水提取工艺。方法:采用紫外法测定壁虎和西洋参中的多糖含量,高效液相色谱(HPLC)法测定喜树果中喜树碱、西洋参中人参皂苷Rb_1的含量;以上述3种成分的提取率及干膏得率、HPLC指纹图谱相似度作为评价指标,通过信息熵赋权法确定各指标权重而计算综合评分;以煎煮时间、加水量、煎煮次数为因素,设计L_9(3~4)正交试验筛选七味蟾参方的最优提取工艺并进行验证试验。结果:最优提取工艺为药材加10倍量水,煎煮3次,每次煎煮1.0 h。验证试验结果显示,在3次提取试验中,多糖、喜树碱、人参皂苷Rb_1平均提取率分别为74.306%、13.860%、52.958%,干膏平均得率为16.150%,指纹图谱相似度平均值为0.991,各指标RSD均小于3.0%(n=3)。结论:优化后的提取工艺重复性好、稳定可行,为该处方的后续开发和产业化生产提供参考依据。

Study on the Optimization of Extraction Technology of Qiwei Chanshen Formula by Orthogonal Test Based on Information Entropy Theory

OBJECTIVE:To optimize the water extraction technology of Qiwei chanshen formula.METHODS:The content of polysaccharides from Gekko japonicus and Panax quinquefolium was determined by UV spectrophotometry,the contents of camptothecin from Camptotheca acuminata and ginsenoside Rb1 from P.quinquefolium were determined by HPLC.The extraction rate of above three components,dry extractum yield and HPLC fingerprint similarity were used as evaluation indicators;information entropy theory was used to determine the weight of each indicator so as to calculate comprehensive score.L9(34)orthogonal test was used to screen the optimal extraction technology of Qiwei chanshen formula with decoction time,water volume and decoction times as factors.Validation test was also performed.RESULTS:The optimal extraction technology included 10-fold water,decocting for 3 times,1.0 h each time.The results of validation test showed that the average extraction rate of polysaccharide,camptothecin and ginsenoside Rb1 were 74.306%,13.860%and 52.958%,respectively.The average dry extractum yield was 16.150%,the average value of fingerprint similarity was 0.991(all RSDs<3.0%,n=3).CONCLUSIONS:The optimized extraction technology is reproducible,stable and feasible,providing reference for the subsequent development and industrial production of the formula.