石菖蒲中生物碱成分的分离与鉴定

目的:分离并鉴定石菖蒲的生物碱成分,为进一步研究其药理活性奠定基础。方法:利用阳离子交换树脂分离石菖蒲的生物碱成分;采用超高效液相色谱串联质谱(UPLC-MS)法进行鉴定,色谱柱为Agilent Zorbax Eclipse Plus-C18,流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),流速为0.3 mL/min,柱温为30℃,进样量为1μL;离子源为电喷雾离子源,采用正离子模式检测,并结合Peakview 1.2等软件对生物碱成分进行分析。结果:石菖蒲中共检测到86个成分,并鉴定出5个生物碱成分,分别为菖蒲碱甲、N-反式阿魏酰酪胺、N-月桂基二乙醇胺、4-羟双氢鞘氨醇和5-丁基尿苷。结论:该试验结果可为石菖蒲生物碱成分的药理活性研究奠定基础。

Isolation and Identification the Compositions of Alkaloids in Acorus tatarinowii

OBJECTIVE:To isolate and identify the compositions of alkaloids in Acorus tatarinowii,and to lay the foundation for further study of its pharmacological activity.METHODS:The compositions of alkaloids from A.tatarinowii was separated by cation exchange resin.UPLC-MS method was used to identify the alkaloids.The separation was performed on a Agilent Zorbax Eclipse Plus-C18 column with mobile phase consisted of 0.1%formic acid-acetonitrile(gradient elution)at a flow rate of 0.3 mL/min.The column temperature was 30℃,and the injection volume was 1μL.ESI ion source was used to detect the fragments,the ion mode scan was positive.Peakview 1.2 and other softwares were used to analyze the compositions of alkaloids.RESULTS:86 compositions were separated and 5 alkaloids were identified,such as tatarine A,N-trans-feruloyltyramine,N-lauryldiethanolamine,phytosphingosine and 5-butyluridine.CONCLUSIONS:The results of the study lay a foundation for the study of pharmacological activity of alkaloids from A.tatarinowii.