人参真菌菌质的乙酸乙酯部位化学成分研究

目的:对生物转化得到的人参真菌菌质的乙酸乙酯部位化学成分进行研究,以期获得活性更好、毒性更低的化合物,为新药研发及人参药材的二次开发利用提供参考。方法:将代号为C-1的真菌种子液加至含有人参的培养基中,生物转化得到人参真菌菌质;称取干燥人参真菌菌质,以70%乙醇溶剂提取浓缩制得稠膏;稠膏加入水混悬,采用乙酸乙酯萃取,获得乙酸乙酯部位。采用薄层色谱、硅胶柱色谱、ODS柱色谱以及半制备液相对上述乙酸乙酯部位进行分离纯化,根据理化性质以及氢谱(~1H-NMR)和碳谱(¹³C-NMR)数据鉴定化合物结构。结果:从人参真菌菌质的乙酸乙酯部位分离、鉴定得到8个化合物,分别为人参皂苷Rs_7(1)、人参皂苷Rk_3(2)、齐墩果酸-28-O-β-D-吡喃葡萄糖苷(3)、人参皂苷Rs_6(4)、20(R)-人参皂苷Rh_1(5)、人参皂苷F_1(6)、三七皂苷R_2(7)、人参皂苷F_4(8)。结论:上述化合物均在人参真菌菌质中发现,其中化合物3、5、6、7、8为经生物转化后得到的,证明生物转化技术能改变人参的化学成分。

Study on the Chemical Constituents of Ethyl Acetate Fraction of Panax ginseng Fungal Substance

OBJECTIVE:To study the chemical constituents of ethyl acetate fraction of Panax ginseng fungal substance obtained by biotransformation,in order to obtain compounds with better activity and lower toxicity,and to provide reference for new drug R&D and the second development and utilization of P.ginseng.METHODS:Fungus of Code Name C-1 seed solution was added into the culture medium containing P.ginseng,and P.ginseng fungal substance was obtained by biotransformation;the dried P.ginseng fungal substance were weighed,extracting with 70%ethanol solvent and concentrating to obtain thick paste.The thick paste was added with water suspension and extracted with ethyl acetate to obtain ethyl acetate fraction.TLC,silica gel column chromatography,ODS column chromatography and semi-prepared liquid phase were used to isolate and purify above ethyl acetate fraction,and the compound structure was identified according to physicochemical properties,hydrogen spectrum(1H-NMR)and carbon spectrum(13C-NMR)data.RESULTS:Eight compounds were isolated and identified from the ethyl acetate fraction of P.ginseng fungal substance and identified as ginsenoside Rs7(1),ginsenoside Rk3(2),oleanolic acid-28-O-β-D-glucopyranoside(3),ginsenoside Rs6(4),20(R)-ginsenoside Rh1(5),ginsenoside F1(6),notoginsenoside R2(7)and ginsenoside F4(8).CONCLUSIONS:All the above compounds were found in P.ginseng fungal substance,which compounds 3,5,6,7 and 8 were obtained after biotransformation,proving that biotransformation technology can change the chemical composition of ginseng.