Alleviation of ischemia/reperfusion injury in ob/ob mice by inhibiting UCP-2 expression in fatty liver |
| |
Authors: | Wan Chi-Dan Wang Chun-You Liu Tao Cheng Rui Wang Hong-Bo |
| |
Institution: | Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China |
| |
Abstract: | AIM: To investigate the protective effect of target suppression of uncoupling protein-2 (UCP-2) on ischemia/ reperfusion (I/R) injury in fatty liver in ob/ob mice.METHODS: Plasmids suppressing UCP-2 expression were constructed, and transfected into fatty liver cells cultured in vitro and the ob/ob mouse I/R injury model. Serum tumor necrosis factor (TNF)-α levels, UCP-2 mRNA expression, alanine aminotransferase (ALT) levels in ob/ob mice were tested, and the pathological changes in fatty liver were observed in experimental and control groups.RESULTS: In ob/ob mouse I/R models, serum TNF-α levels were significantly higher than in normal controls. After the plasmids were transfected into the cultured cells and animal models, expression of UCP-2 mRNA was significantly reduced as compared with that in the control group (2156±015 vs 2"0-45±0-15, P < 0.05). In ob/ob mouse models, in which expression of UCP-2 was suppressed, serum ALT levels were significantly lower than those of other groups, and pathological analysis revealed that injury of liver tissues was significantly alleviated.CONCLUSION: The target suppression of UCP-2 expression in fatty liver can alleviate the I/R injury in the ob/ob mice. |
| |
Keywords: | ob/ob mice Fatty liver Uncoupling protein-2 Ischemia/reperfusion injury |
本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录! |
|