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| 白细胞介素-13上调巨核细胞系-Dami细胞原癌基因c-mpl的研究 | |
| 引用本文： | 李克强,李文林,石小玉,彭淑牖,刘颖斌,刘宏.白细胞介素-13上调巨核细胞系-Dami细胞原癌基因c-mpl的研究[J].中国病理生理杂志,2003,19(9):1170-1173. |
| 作者姓名： | 李克强 李文林 石小玉 彭淑牖 刘颖斌 刘宏 |
| 作者单位： | 1. 浙江大学医学院第二附属医院普外科, 浙江 杭州 310009; 2. 江西医学院病理生理学教研室, 江西省医学生物高技术重点实验室, 江西 南昌 330006; 3. 江西医学院形态学中心实验室, 江西 南昌 330006 |
| 基金项目： | 国家自然科学基金资助项目 (No .3986 0 0 33)，江西省自然科学基金资助项目 (No.984 0 17，No .0 14 0 0 36 ) |
| 摘 要： | 目的:研究重组人白细胞介素-13(rhIL-13)对巨核细胞系-Dami细胞原癌基因c-mpl表达的影响。方法:用RT-PCR法检测c-mplmRNA的表达。用配体结合实验检测膜蛋白c-mpl的表达。结果:经25μg/LrhIL-13处理过的实验组Dami细胞c-mplmRNA的表达比对照组高24.8%。经100μg/LrhIL-13处理过的实验组Dami细胞膜蛋白c-mpl的表达比对照组高28.5%(P＜0.05)。结论:rhIL-13增强了Dami细胞原癌基因c-mpl表达;膜蛋白c-mpl与促血小板生成素(TPO)的结合功能正常;rhIL-13促进Dami细胞增殖的部分机制可能是通过上调原癌基因c-mpl的表达来实现的。 |
| 关 键 词： | 白细胞介素13 重组 Dami细胞 原癌基因c-mpl 血小板生成素 |
| 文章编号： | 1000-4718(2003)09-1170-04 |
| 收稿时间： | 2002-07-17 |
| 修稿时间： | 2002年7月17日 |
Upregulation of proto-oncogene c-mpl by interleukin-13 in Dami cells |
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| LI Ke-qiang ,LI Wen-lin ,SHI Xiao-yu ,PENG Shu-you ,LIU Ying-bin ,LIU Hong.Upregulation of proto-oncogene c-mpl by interleukin-13 in Dami cells[J].Chinese Journal of Pathophysiology,2003,19(9):1170-1173. | |
| Authors: | LI Ke-qiang LI Wen-lin SHI Xiao-yu PENG Shu-you LIU Ying-bin LIU Hong |
| Institution: | 1. Department of General Surgery, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China; 2. Department of Pathophysiology, Jiangxi Key Laboratory of Medical Biotechnology, Nanchang 330006, China; 3. Central Laboratory of Morphology, Jiangxi Medical College, Nanchang 330006, China |
| Abstract: | AIM:To investigate the effects of recombinant human interleukin-13 (rhIL-13) on the expression of proto-oncogene c-mpl in Dami cells, a human megakaryobiastic leukemia cell line.METHODS:The expression of c-mpl mRNA in Dami cells was investigated with RT-PCR. The expression of membrane-bound protein c-mpl on Dami cells was investigated with ligand combination experiment.RESULTS:In RT-PCR experiment, we found the quantitis of expression of c-mpl mRNA in 25 μg/L rhIL-13 group increased by 24.8%. In ligand combination experiment, we found quantitis of expression of membrane-bound protein c-mpl in 100 μg/L rhIL-13 group increased by 28.5% (P＜0.05).CONCLUSION:Our data indicate that rhIL-13 enhances the expression of proto-oncogene c-mpl in Dami cells. Membrane-bound protein c-mpl on the surface of Dami cells bind to TPO (thrombopoietin) normally, moreover, the proliferation of Dami cells stimulated by rhIL-13 may be partly due to increasing the proto-oncogene c-mpl expression in Dami cells. |
| Keywords: | Interleukin-13 recombinant Dami cell Proto-Oncogene c-mpl Thrombopoietin |
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