A monoclonal antibody reactive with normal and leukemic human myeloid progenitor cells |
| |
| Authors: | J D Griffin D Linch K Sabbath P Larcom S F Schlossman |
| |
| Affiliation: | Division of Tumor Immunology and Pediatric Oncology, Dana-Farber Cancer Institute, the Division of Hematology and Oncology, Children''s Hospital Medical Center and the Department of Medicine, Harvard Medical School, Boston, MA 02115, U.S.A. |
| |
| Abstract: | Anti-MY9 is an IgG2b murine monoclonal antibody selected for reactivity with immature normal human myeloid cells. The MY9 antigen is expressed by blasts, promyelocytes and myelocytes in the bone marrow, and by monocytes in the peripheral blood. Erythrocytes, lymphocytes and platelets are MY9 negative. All myeloid colony-forming cells (CFU-GM), a fraction of erythroid burst-forming cells (BFU-E) and multipotent progenitors (CFU-GEMM) are MY9 positive. This antigen is further expressed by the leukemic cells of a majority of patients with AML and myeloid CML-BC. Leukemic stem cells (leukemic colony-forming cells, L-CFC) from most patients tested were also MY9 positive. In contrast, MY9 was not detected on lymphocytic leukemias. Anti-MY9 may be a valuable reagent for the purification of hematopoietic colony-forming cells and for the diagnosis of myeloid-lineage leukemias. |
| |
| Keywords: | Myeloid surface antigen leukemia diagnosis CFU-GM leukemic colony-forming cells granulocyte and monocyte colony-forming cell acute myeloblastic leukemia chronic myeloid leukemia in blast crisis acute lymphoblastic leukemia chronic lymphocytic leukemia sheep erythrocyte rosetting cells fluorescence-activated cell sorting erythroid burst-forming unit erythroid colony-forming unit Iscove's modified Dulbecco's minimal essential medium mixed colony-forming cell complement acute nonlymphoblastic leukemia terminal deoxynucleotidyl transferase common acute lymphoblastic leukemia antigen leukemic colony-forming cell |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
