干扰素治疗婴幼儿轮状病毒肠炎临床疗效及免疫功能变化

目的探讨轮状病毒肠炎的免疫学发病机制,并观察IFN治疗婴幼儿轮状病毒肠炎后临床疗效及免疫功能变化。方法 140例轮状病毒(HRV)肠炎患儿随机分为治疗组常规治疗,观察组加用干扰素(IFN),观察两组患儿治疗后临床疗效及免疫功能变化。血清免疫球蛋白、T淋巴细胞亚群、IL-2、sIL-2R、IL-10测定分别采用单向免疫扩散法、APAAP法、ELISA法和双抗夹心ELISA法。结果治疗前本组患儿与对照组比较IgA、IgG、lgM、CD4、CD4/CD8、IL-2和IL-10活性显著下降(P均<0.01),CD8和sIL-2R显著升高(P均<0.01)。干扰素治疗后观察组总有效率85.7%明显高于治疗组57.1%(P<0.01),且观察组免疫功能较治疗前明显改善,差异具有统计学意义(P<0.01)。结论提示轮状病毒肠炎患儿免疫功能低下且紊乱,IFN有助于提高疗效以及恢复其机体免疫功能。

Clinical curative effect of interferon on children'rotavirus enteritis and observation of immtme function

Objective Examined interleukin-10(IL-10),IL-2 serum soluble IL-2 receptor(sIL-2R) levels and T-lymphocyte subsets in peripheral blood of 70 childhood and analysed its immnunological pathogenesis as well as observed interferon treatment rotavirus enteritis efficiency.Methods 140 cases with human rotavirus(HRV) enteritis were randomly divided into two groups after the detection of immunoglobulin,T-cell groups IL-10、IL-2 and sIL-2R.One group was given interferon apart from the conventional treatment and another group was selected as the control,serum immunoglobulin,IL-10,IL-2 and sIL-2R as well as T lymphocyte subsets were assayed by double antibody sandwich ELISA,ELISA and APAAP methods.Results IgA,IgG,IgM,CD4,CD4/CD8,IL-2 and IL-10 were very low but CD8 and sIL-2R very high before the treatment of interferon in both groups(P<0.01).After the treatment,IgA,IgG,IgM,CD4and CD4/CD8,IL-2 and IL-10 prominently increased,but CD8 and sIL-2R remarkably decreased in the tested group(P<0.01).The effective rate in the tested group(85.7%) is much higher than that in the control group(57.1%) with P<0.01.Conclusion The results suggest that a immune functional disorder may exist in HRV enteritis and the treatment with interferon will shorten the period of the disease and improve the immune function of the patients and less side effects,worthy of clinical application.