| 桑葚中多糖的提取及含量测定 |
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| 引用本文： | 刘玉玲,纪国力. 桑葚中多糖的提取及含量测定[J]. 中国医药科学, 2012, 0(18): 109-110 |
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| 作者姓名： | 刘玉玲 纪国力 |
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| 作者单位： | 山东省泰安市食品药品检验中心 |
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| 摘 要： | 目的研究桑葚中多糖的提取及含量测定方法。方法采用超声提取技术提取桑葚中的多糖,并以苯酚-硫酸法测定其含量。结果葡萄糖在0.01～0.06mg/mL范围内,其浓度与吸光度的线性关系良好,回归方程为A=7.2346C-0.0081(r=0.9997),平均回收率为98.92%(n=6,RSD=3.50%),桑葚中多糖含量为9.42%。结论该方法操作简单,灵敏度高,测定结果可靠,可用于桑葚中多糖含量的测定。
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| 关 键 词： | 桑葚 苯酚-硫酸法 多糖 |
Extraction and content determination of polysaccharides from Fructus Mori |
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| LIU Yuling,JI Guoli. Extraction and content determination of polysaccharides from Fructus Mori[J]. China Medicine and Pharmacy, 2012, 0(18): 109-110 |
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| Authors: | LIU Yuling JI Guoli |
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| Affiliation: | Taian Municipal Testing Center for Food and Drugs,Taian 271000,China |
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| Abstract: | Objective To study the extraction and determination of polysaccharides from Fructus Mori. Methods The polysaccharide was extracted by ultrasonic extraction technique,and the polysaccharide content was determined by phenol- sulfuric acid method. Results The linear relationship of concentration and absorbance of glucose was 0.01-0.06 mg/mL,and the regression equation was A=7.234 6C-0.008 1(r=0.999 7),the average recovery was 98.92%(n=6,RSD=3.50%),and the polysaccharide content of Fructus Mori was 9.42%. Conclusion This method is simple,high sensitivity and reliable,which can be used for the determination of polysaccharides inFructus Mori. |
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| Keywords: | Fructus Mori Phenol-sulfuric acid method Polysaccharide |
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