| 地塞米松诱导人小梁细胞bcl—2基因的表达 |
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| 引用本文： | 卓业鸿 葛坚. 地塞米松诱导人小梁细胞bcl—2基因的表达[J]. 眼科学报, 1998, 14(4): 187-189 |
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| 作者姓名： | 卓业鸿 葛坚 |
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| 作者单位： | 中山医科大学中山眼科中心，中山医科大学中山眼科中心，中山医科大学中山眼科中心，中山医科大学中山眼科中心，中山医科大学中山眼科中心 广州 510060，广州 510060，广州 510060，广州 510060，广州 510060 |
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| 基金项目： | 国家自然科学基金(编号39770789,39800163)，广东省科委基金(编号980112) |
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| 摘 要： | 目的:探讨地塞米松对小梁细胞bcl-2基因表达的影响。方法:应用组织块培养的方法建立人小梁细胞培养。取第三代小梁细胞培养于载玻片上,含10~(-7)mol/L地塞米松的培养液作用6小时、12小时、24小时后,应用LSAB观察bcl-2基因表达的情况。结果:地塞米松作用6小时后可见bcl-2蛋白阳性细胞,且随时间的延长,阳性细胞有所增多。结论:地塞米松可诱导小梁细胞bcl-2基因表达,可能在激素性青光眼发病中起着一定的作用。眼科学报1998;14:187～189。
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| 关 键 词： | 地塞米松 小梁细胞 bcl-2基因 |
Expression of bcl-2 Gene in Human Trabecular Cells Induced by Dexamethasone |
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| Yehong Zhuo,Jian Ge,Minkai Linjianliang Zheng,Yanyan LiZhongshan Ophthalmic Center,Sun Yat-sen University of Medical Sciences,Guangzhou ,China. Expression of bcl-2 Gene in Human Trabecular Cells Induced by Dexamethasone[J]. Eye science, 1998, 14(4): 187-189 |
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| Authors: | Yehong Zhuo Jian Ge Minkai Linjianliang Zheng Yanyan LiZhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China |
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| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. |
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| Abstract: | Purpose:To investigate the influence of dexamethasone on expression of bcl-2 gene in human trabecular cells.Methods: The trabecular specimens from human eyes were cultured in vitro and the cultured cells of the third generation on slides were immersed in culture solution containing 10-7M dexamethasone for 6 hours, 12 hours and 24 hours respectively. The expression of bcl-2 gene was studied with LSAB.Results: The positive cells of the bcl-2 protein could be identified after dexamethasone effected for 6 hours and the positive cell increased with time going on. Conclusion :The expression of bcl-2 gene can be induced by dexamethasone, it may be related with glaucomatic pathogenesis. Eye Science 1998 ; 14 ; 187 - 189. |
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| Keywords: | dexamethasone trabecular cells bcl-2 gene |
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