眼部淋巴组织增生性病变PCNA和AgNORs的观察

目的：探讨眼部淋巴组织增生性病变的增殖活性与组织学分型的关系。方法：应用抗增殖细胞核抗原的单克隆抗体ＰＣ１０和ＡｇＮＯＲｓ技术，对３４例眼附属器淋巴组织增生性病变（包括良性淋巴组织增生１４例，不典型增生９例，恶性淋巴瘤１１例）的石腊切片进行检测。结果：良性淋巴组织增生与后二者ＰＣＮＡ及ＡｇＮＯＲｓ计数之间均有非常显著的差异（ＰＣＮＡ Ｐ〈０．００１，ＡｇＮＯＲｓＰ〈０．０１及Ｐ〈０．００１），不典

The observation of proliferative cell nuclear antigen and AgNORs in ocular adnexal lymphoid hyperplasia]

PURPOSE: To investigate the expression of proliferative cell nuclear antigen and Nuclear Organizer Region in ocular adnexal lymphoid hyperplasia and its histopathologic classification. METHOD: Using anti-proliferative cell nuclear antigen monoclonal antibody (PC10) and AgNORs method, the proliferative cell in 34 cases of ocular adnexal lymphoid neoplasms (benign lymphoid lesion, BLL, 14 cases; atypical lymphoid hyperplasias, AH, 9 cases; malignant lymphomas, ML, 11 cases) were detected in formalin fixed and paraffin-embeded tissue. RESULTS: A significant difference between BLL and AH, BLL and ML was observed (PCNA P < 0.001, P < 0.001; AgNORs P < 0.01, P < 0.001); similar result was observed between AH and ML in PCNA index (P < 0.01). But there was not a significant difference in AgNORs count (P > 0.05). The linear regression coefficient between PCNA index and AgNORs number was excellent (0.8693). CONCLUSION: The two methods may be helpful to distinguish ocular adnexal lymphoid reactive hyperplasia and malignant lymphoma.