喉鳞状细胞癌原发灶和淋巴结转移灶中PTEN和Survivin的表达及意义

目的:探讨喉鳞状细胞癌(LSCC)原发灶与淋巴结转移灶在分子水平的差别及意义。方法:采用免疫组织化学SP法检测18例转移淋巴结阳性(N )的LSCC患者,对其原发灶、转移灶及无转移淋巴结中PTEN和Survivin的表达进行检测;另取22例声带息肉(VCP)组织标本作为对照组。结果:无转移淋巴结患者的标本中均未见PTEN和Survivin的表达。LSCC、N 、VCP中PTEN阳性率分别为94.4%(17/18)、94.4%(17/18)和95.5%(21/22),3者阳性率比较,差异无统计学意义。N 的PTEN表达强度较LSCC为强,其表达强度差异有统计学意义(P<0.05);Survivin在VCP中未见阳性表达,在LSCC和N 中阳性率分别为55.6%(10/18)、50.0%(9/18),三者阳性率比较差异有统计学意义(P<0.01),但LSCC和N 中的阳性表达率和表达强度三组比较均无统计学意义(P<0.05)。结论:LSCC原发灶与淋巴结转移灶中分子水平的特点存在差异。

Expression and significance of PTEN and Survivin between the primary foci and the lymph node metastases foci of laryngeal squamous cell carcinoma

OBJECTIVE: To investigate the molecular difference and significance between the primary foci and metastases foci of laryngeal squamous cell carcinoma (LSCC). METHOD: Tissues of 18 cases of positive lymph node metastases (N+) LSCC were detected by using SP method. The LSCC primary foci and metastases foci and negative metastases lymph nodes were included, 22 cases of vocal cord polyp (VCP) were regarded as control. expression of PTEN and Survivin were evaluated by SP immunohistochemistry. RESULT: Expression of neither PTEN nor Survivin were positive in negative nodes. The positive rates of PTEN in LSCC, N+, VCP were 94.4% (17/18), 94.4% (17/18) and 95.5% (21/22) respectively, there were no significant difference among them (P>0.05). But the expression degree were more powerful in N+ than in the LSCC (P<0.05). There was no expression of Survivin in VCP. The positive rates of Survivin in LSCC and N+ were 55.5% (10/18), 50.0% (9/18), the difference were significant (P<0.01); but between LSCC and N+, neither the positive rate nor the expression degree had significant difference (P>0.05). CONCLUSIONS: There were differences between the LSCC primary foci and the lymph node metastases foci in molecular level probably.