HepG2细胞胰岛素抵抗模型的建立及在筛选桑叶有效部位中的应用

目的体外建立HepG2细胞胰岛素抵抗模型，并用于筛选桑叶防治胰岛素抵抗活性的有效部位。方法采用高浓度胰岛素诱导HepG2细胞建立肝胰岛素抵抗模型，研究桑叶有效部位对胰岛素抵抗模型细胞葡萄糖消耗的影响。结果将HepG2细胞置于10 μg·mL－1胰岛素中48 h，HepG2细胞对胰岛素的抵抗作用最明显，其特性可维持48 h。桑叶水提部位能促进HepG2胰岛素抵抗模型的葡萄糖消耗。结论高胰岛素诱导培养法可以复制出稳定可靠的肝胰岛素抵抗细胞模型。桑叶水提物、多糖、黄酮均可以促进葡萄糖的吸收。

Establishment of Insulin Resistant HepG2 Cell Model and Its Application in Screening Bioactive Components of Mulberry Leaves

Objective To establish an insulin resistant HepG2 cell model in vitro to screen bioactive components of mulberry leaves.Methods The insulin resistant HepG2 cell model was induced by high concentration of insulin.Glucose consumption of the cells was detected after administration with the bioactive components of the mulberry leaves.Results After the HepG2 cells were cultured with insulin(10 μg·mL-1) for 48 h,insulin resistance of the cells was the most apparent and maintained for 48 h.The bioactive components of mulberry leaves could promote glucose consumption of insulin resistant HepG2 cell model.Conclusion The insulin resistant HepG2 cell model was successfully established in vitro by high concentration of insulin.Extracts,polysaccharides and flavonoids of the mulberry leaves enhance glucose absorption.