干细胞因子和肥大细胞在慢性肾衰竭大鼠皮肤中的表达和浸润

目的 探讨干细胞因子(SCF)和肥大细胞(MC)与慢性肾衰竭大鼠皮肤损害发生发展的关系.方法 36只雄性Wistar大鼠按随机数字表法分为两组:模型组(n=18)用腺嘌呤灌胃诱发大鼠慢性肾衰竭,对照组(n=18)用等量生理盐水灌胃.分别于实验第4、8和12周各组随机处死6只大鼠,甲苯胺蓝染色观察皮肤组织中MC的浸润,免疫组织化学方法和实时荧光定量PCR检测皮肤组织中SCF蛋白和mRNA的表达量,并分析它们之间的相关性.结果 从第8周开始,模型组大鼠皮肤组织中MC密度和SCF的表达均显著高于对照组(P＜0.01),且2者均随着灌胃天数的延长逐渐增加.模型组MC密度与SCF蛋白及mRNA的表达量均呈正相关(r分别为0.81、0.65,均P＜0.01).结论 慢性肾衰竭时皮肤组织中MC的浸润和SCF的表达均显著增多,提示2者可能参与慢性肾衰竭皮肤损害的发生发展.

Expression of stem cell factor and infiltration of mast cells in dermal tissue of rats with chronic renal failure

Objective To investigate the role of stem cell factor(SCF) and mast cells(MC) in the pathogenesis and progression of dermal lesions caused by chronic renal failure． Methods Thirty-six Wistar rats were randomly divided into model group (adenine lavage at a dose of 150 mg•kg-1•d-1) and control group(physiological saline lavage at equal volume)． Six rats from each group were sacrificed respectively at week 4, 8 and 12． The intensity of MC infiltration was examined by toluidine blue staining. The expression of SCF was detected by immunohistochemistry and real-time fluorescence quantitative PCR． Results Compared with control group, the intensity of MC and the expression of SCF were significantly higher in dermal tissue of model group (P＜0.01, respectively), and they were increased with time． In the model group，the number of MC infiltration was positively correlated with both the protein expression of SCF (r=0.81, P＜0.01) and the level of SCF mRNA (r＝0.65, P＜0.01)． Conclusion The increased SCF and MC may participate in the pathogenesis and progression of dermal lesions caused by chronic renal failure．