通心络对小鼠反复缺氧耐受性的影响

目的 观察通心络对反复缺氧小鼠缺氧耐受性的作用及其机制.方法 雄性昆明小鼠80只按体重分为2组,每组40只.通心络组(通心络超微粉混悬液按0.01 ml/g体重灌胃).反复缺氧组(对照组,用0.5%羧甲基纤维素钠溶液按0.01 ml/g体重灌胃).两组动物分别进行缺氧前及重复缺氧暴露、缺氧1次(H1)、3次(H3)、5次(H5),观察小鼠的缺氧耐受时间,利用蛋白质印迹法检测脑组织缺氧诱导因子-la(HIF-la)、血管内皮生长因子(VEGF)蛋白的表达.结果 两组小鼠随着缺氧次数的增加,缺氧耐受时间依次增长,HIF-la、VEGF蛋白表达逐次增强.与对照组比较,通心络组小鼠在缺氧1、3、5次H1(18.0±2.4)min比(15.6±2.0)min、H3(68.3±13.2)min比(41.7±9.0)min、H5(85.9±7.0)min比(51.4±14.4)min]中明显增长(P<0.01或P<0.05);脑组织HIF-la在H1(0.95±0.04比0.79±0.02)、H3(1.01±0.03比0.85±0.02)、H5(1.16±0.02比0.92±0.03)中蛋白表达均明显增强(P<0.01或P<0.05);脑组织VEGF在H3(1.14±0.02比0.89±0.03)、H5(1.34±0.05比0.99±0.07)中蛋白表达均明显增强(P<0.01或P<0.05).结论 HIF-la、VEGF表达增加可能是机体自适应的机制之一,反复缺氧显示出机体具有强大的自适应能力,通心络能够明显提高反复缺氧小鼠的自适应能力.

Effects of Tongxinluo on repeated hypoxic tolerance in mice

Objective To observe the effects of Tongxinluo (TXL) on repeated hypoxic tolerance in mice and explore the underlying mechanisms. Methods Mice were randomly divided into groups of repeated hypoxia (control) and TXL according to body weights. The mice in each group were exposed to acute repeated hypoxia for 0 run ( H0 ) , 1 run ( H1 ) , 3 runs ( H3 ) and 5 runs ( H5 ) . The animal's tolerance time of each hypoxic exposure was recorded. Western blot was used to measure the protein levels of hypoxia inducible factor-la (HIF-la) and vascular endothelial growth factor (VEGF) in cortex tissue. Results The hypoxic tolerance time in control and TXL groups significantly increased run by run. Both HIF-la and VEGF proteins in two groups increased gradually. Compared with control group, the tolerance time in H1 of TXL group (18. 0±2. 4) min vs (15.6±2.0 )min] , H3 (68. 3±13. 2) min vs (41.7±9.0) min) and H5( 85.9±7.0) min vs (51.4±14.4) min] increased (P<0.05 or P <0.01); the HIF-la protein expression in H1 of TXL group (0. 95±0. 04 vs 0.79±0. 02) , H3 (1. 01±0. 03 vs 0. 85±0.02), H5(l. 16±0.02 vs 0.92±0.03) increased (P<0. 05 or P<0. 01) ; the VEGF protein expression in H3 of TXL group (1. 14±0. 02 vs 0. 89±0.03), H5( 1. 4±0.05 vs 0.99±0. 07) increased (P <0. 05 or P <0. 01). Conclusions Under repeated hypoxia, an organism has a strong daptive ability. The rises of HIF-la and VEGF may be an adaptive mechanism. TXL can increase obviously the adaptive ability of mice to hypoxia.