脂联素单核苷酸多态性+45位点的表达在2型糖尿病中的研究

目的研究脂联素单核苷酸多态性SNP＋45位点基因在广州汉族2型糖尿病患者中的分布，评价该基因是否为2型糖尿病的易感基因。方法采用聚合酶链式反应-限制性内切酶长度多态性技术，对2型糖尿病（T2DM）组和健康对照（CON）组人群进行脂联素基因SNP＋45多态位点基因的检测。结果T2DM组人群脂联素SNP＋45位点的基因型分布：TT型56．4％、GT型34．9％、GG型8．7％。CON组脂联素SNP＋45位点的基因型分布：TT型42．0％、GT型48．2％、GG型9．8％。结论广州地区T2DM组与CON组脂联素SNP＋45位点的基因型差异有统计学意义。T2DM组T／T型基因明显增多；CON组GT＋GG型基因增多。提示脂联素基因第2外显子45位点多态性与2型糖尿病相关，TT基因型者具有较高的2型糖尿病易感性。

Research on adiponectin's single nucleotide polymorphisms(SNP+45)site expression in type 2 diabetes mellitus

Objective To investigate the distribution of the adiponectin's single nucleotide polymorphism sites(SNP+ 45)in patients with type 2 diabetes mellitus in Guangzhou, and to evaluate whether the site gene is the susceptible genes of type 2 diabetes mellitus. Methods To detect mononucleotide polymomhism sites of SNP45 between control group and type 2 diabetes mellitus group with PCR-restriction endonuclease length polymorphism test. Results The genotype distribution of SNP+.45 in type 2 diabetes mellitus group were as follows:TT 56.4% ,GT 34.9% ,GG 8.7%. The genotype distribution of SNP+45 in control group were as follows:TT 42.0% ,GT 48.2% ,GG 9.8%. Conclusion According to the results,difference of SNP+45 site geneotype between type 2 diabetes mellitus group and control group is significant in Guangzhou. It means the second exon polymorphism of SNP+45 associated with type 2 diabetes mellitus. TT type is susceptible to type 2 diabetes mellitus.