| 奥沙利铂对 K562细胞体外生长的影响 |
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| 引用本文： | 陈姝,汤为学,娄世锋. 奥沙利铂对 K562细胞体外生长的影响[J]. 重庆医科大学学报, 2004, 29(4): 452-454,458 |
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| 作者姓名： | 陈姝 汤为学 娄世锋 |
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| 作者单位： | 重庆医科大学第二临床学院血液科,重庆,400010;重庆医科大学病理生理教研室,重庆,400016 |
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| 摘 要： | 目的:观察奥沙利铂对人白血病K562细胞的作用.方法:检测奥沙利铂对K562细胞作用的时间效应和剂量效应,在电镜下观察奥沙利铂作用后细胞形态变化;用流式细胞仪分析细胞周期的改变.结果:随着作用时间及药物浓度的增加,奥沙利铂对K562细胞的增殖抑制作用就越明显.0.016mmol/L奥沙利铂作用72h,K562细胞生长抑制率达85%,形态学及流式细胞仪分析显示G2/M期阻滞和细胞凋亡.结论:奥沙利铂诱发的人白血病细胞的凋亡与细胞分裂期阻滞密切相关.
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| 关 键 词： | 奥沙利铂 白血病 细胞凋亡 |
| 文章编号： | 0253-3626(2004)04-0452-03 |
Growth-inhibiting effects of oxaliplatin on K562 cells in vitro |
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| CHEN Shu,et al. Growth-inhibiting effects of oxaliplatin on K562 cells in vitro[J]. Journal of Chongqing Medical University, 2004, 29(4): 452-454,458 |
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| Authors: | CHEN Shu et al |
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| Abstract: | Objective:To investigate the effects of oxaliplatin on human leukemia K562 cell and its mechanism.Methods:The leukemia cell growth was measured by MTT-microculture tetrazolium assay.Cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination.Results:Oxaliplatin inhibited the leukemia cell growth in concentration- and time-dependent manners.Marked cells accumulated in G_2/M phase.The rate of cell growth inhibition was 85% after intervention in 0.016 mmol/L oxaliplatin for 72 hours.The cells showed G_2/M arrest and apoptosis.Conclusion:Oxaliplatin suppresses the growth of K562 cells in vitro by causing cell-cycle arrest,and cell apoptosis.The mechanisms of oxaliplatin provide theoretical guidance for clinical treatment. |
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| Keywords: | Oxaliplatin Leukemia Apoptosis |
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